Standard molecular biology-based methods were utilized for DNA manipulations. DNA modifying enzymes and restriction nucleases (Promega and New England Biolabs), Gibson assembly mix (New England Biolabs), Phusion DNA Polymerase (New England Biolabs), and Taq DNA polymerase (Promega) were used following the manufacturer’s instructions. All modified DNA fragments were tested by colony PCR and verified by Sanger sequencing (Eurofins).
Dna modifying enzymes and restriction nucleases
Manufactured by Promega
DNA modifying enzymes and restriction nucleases are laboratory tools used for manipulating DNA. These enzymes facilitate specific modifications to DNA sequences, such as cutting, joining, or altering the genetic material. They are essential for various molecular biology techniques like cloning, gene editing, and genetic analysis.
Automatically generated - may contain errors
Lab products found in correlation
Gibson assembly mix, by New England Biolabs (2 mentions)
Sanger sequencing, by Eurofins (2 mentions)
Phusion dna polymerase, by New England Biolabs (1 mentions)
Taq dna polymerase, by Promega (1 mentions)
Phusion, by New England Biolabs (1 mentions)
Onetaq dna polymerase, by New England Biolabs (1 mentions)
2 protocols using dna modifying enzymes and restriction nucleases
1
DNA Manipulation and Verification
2
Construction of Mutants in V. cholerae
Check if the same lab product or an alternative is used in the 5 most similar protocols
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In-frame deletions and promoter-replacement mutants in V. cholerae were constructed by previously described allelic exchange methods (32 (link)). Standard molecular biology-based methods were utilized for DNA manipulations. DNA-modifying enzymes and restriction nucleases (Promega and New England Biolabs); Gibson assembly mix (New England Biolabs); and Q5, Phusion, and OneTaq DNA polymerases (New England Biolabs) were used according to the manufacturer’s instructions. All recombinant DNA constructs were tested by PCR and verified by Sanger sequencing (Eurofins).
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