Control, shSTAG2-1, and shSTAG2-2 T24 and HB-CLS-1 cells were plated at 60% confluence in 12-well plates. Twenty hours later, each plate was imaged for 30 hours (time-lapse 1 image per 5 minute) using a motorized-staged environment-controlled Leica AF6000 Live Cell Imaging System. Each condition was imaged in duplicate. Motility of cells was estimated by time-lapse monitoring of their trajectories. Tracks of individual cells were determined from the series of changes in cell centroid positions (30 hours with 5-minute intervals), pooled and analyzed as described previously (37 (link)).
Af6000 live cell imaging system
Manufactured by Leica
The AF6000 Live Cell Imaging System is a high-performance microscope platform designed for live-cell imaging applications. It provides advanced features for capturing real-time, high-resolution images and videos of living cells. The system combines state-of-the-art optics, automated hardware, and integrated imaging software to enable researchers to observe and analyze dynamic cellular processes with precision.
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Lab products found in correlation
2 protocols using af6000 live cell imaging system
1
Time-lapse Imaging of Cell Motility
2
Time-lapse Monitoring of Cell Motility
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