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Sodium alginate alg

Manufactured by FMC Biopolymer
Sourced in Norway

Sodium alginate (ALG) is a naturally derived biopolymer extracted from brown seaweed. It is a water-soluble, viscous polysaccharide that can be used as a stabilizer, thickener, and gelling agent in various applications.

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2 protocols using sodium alginate alg

1

Hyaluronate-Alginate Hydrogel Synthesis

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Sodium hyaluronate was purchased from Lifecore
(Mw = 700, 1500, 2500 kDa; Chaska, MN)
and Humedix (Mw = 1000 kDa; Anyang, Korea).
Sodium alginate (ALG) (Mw = 250 kDa, guluronate
unit content = 66%) was purchased from FMC Biopolymer (Sandvika, Norway).
RGD peptide (GGGGRGDSP) and HAV peptide (GGGGSHAVSS) were purchased
from Anygen (Seoul, Korea). 1-Ethyl-3-(dimethylaminopropyl)carbodiimide
(EDC) hydrochloride was purchased from Proteochem (Hurricane, UT). N-Hydroxysulfosuccinimide sodium salt (sulfo-NHS) was purchased
from Covachem (Loves Park, IL). Adipic acid dihydrazide (ADH), 2-(N-morpholino)ethanesulfonic acid (MES) hydrate, calcium
sulfate, deuterium oxide (D2O), Alcian blue, and direct
red 80 were purchased from Sigma-Aldrich (St. Louis, MO). Dulbecco’s
phosphate-buffered saline (DPBS), fetal bovine serum (FBS), Dulbecco’s
modified Eagle’s medium nutrient mixture F-12 (DMEM/F-12),
penicillin–streptomycin (P–S), and trypsin–ethylenediaminetetraacetic
acid (EDTA) were procured from Gibco (Grand Island, NY). The Ez-Cytox
kit was purchased from DoGenBio (Daejeon, Korea), and the Live/Dead
Viability/Cytotoxicity kit was purchased from Invitrogen (Waltham,
MA).
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2

Whey Protein Isolate Characterization

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Journal Pre-proof
Whey protein isolate (WPI) BiPRO® (98% w/w) mainly consisting of 65% β-lactoglobulin, 25%
α -lactoglobulin and 8% bovine serum albumin was kindly provided by Davisco Foods International Inc., (Le Sueur, MN, USA). The molecular weight and mean square radius of gyration (R g ) of WPI are 2.0 10 4 Da and 11 nm, respectively. Sodium alginate (ALG) with a weight-average × molecular mass of Mw = 2.3 10 5 Da and R g = 78.8 nm (100% w/w) was obtained from FMC × BioPolymer (Norway). D-glucono-δ-lactone (GDL) was purchased from Aladdin Chemistry (China).
All other chemicals were of analytical grade. Ultrapure water of Milli-Q system (18.3 MΩ.cm) was used for sample preparation.
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