Gas permeable membrane

Manufactured by Avantor

Sourced in United States

Gas-permeable membranes are thin, porous materials that allow the passage of gases, such as oxygen and carbon dioxide, while retaining other substances. They serve as a barrier to protect against contaminants and maintain desired gas concentrations in controlled environments.

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Lab products found in correlation

Ht multitron, by Infors (1 mentions)

2 protocols using gas permeable membrane

Fitness Profiling of P. putida Transposon Library

Cited 4 times

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As described in prior reports (72 (link)), the P. putida KT2440 RB-TnSeq library, JBEI-1, was thawed, inoculated into 25 mL of Luria broth (LB) supplemented with 50-µg/mL kanamycin, and grown to OD₆₀₀ of 0.5. Three 1-mL samples were taken after this step to serve as t₀ records of barcode abundance. The library was then washed via centrifugation and resuspension in an equal volume of MOPS [3-(N-morpholino)propanesulfonic acid] minimal medium (MM) (Table S4). The washed cells were then diluted 1:50 in MOPS MM with 10 mM L-glutamate serving as the sole carbon source. The library was cultured in a 96-well deep well plate sealed with a gas-permeable membrane (VWR, USA). The plate was shaken (700 rpm) in an INFORS HT Multitron (Infors USA Inc.) at 30°C for 24 hours. Duplicate 600-µL samples were then combined, and BarSeq analysis was conducted as described previously (73 (link)– (link)75 (link)). Single carbon source fitness data are available at http://fit.genomics.lbl.gov (48 (link)).

Garber M.E., Frank V., Kazakov A.E., Incha M.R., Nava A.A., Zhang H., Valencia L.E., Keasling J.D., Rajeev L, & Mukhopadhyay A. (2023). REC protein family expansion by the emergence of a new signaling pathway. mBio, 14(6), e02622-23.

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Characterizing P. putida KT2440 Fitness by RB-TnSeq

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The P. putida KT2440 RB-TnSeq library, JBEI-1 47 , was thawed, inoculated into 25 mL of LB medium supplemented with kanamycin, and grown to OD600 of 0.5. Three 1 mL samples were taken after this step to serve as t0 records of barcode abundance. The library was then washed via centrifugation and resuspension in an equal volume of MOPS minimal medium. The washed cells were then diluted 1:50 in MOPS minimal medium with 10 mM L-glutamate serving as the sole carbon source. The library was cultured in a 96-well deep well plate sealed with a gas permeable membrane (VWR, USA). The plate was shaken (700 rpm) in an INFORS HT Multitron (Infors USA Inc.) at 30 °C for 24 hrs. Duplicate 600 μL samples were then combined and BarSeq analysis was conducted as described previously [48] [49] [50] .
Single carbon source fitness data is available at http://fit.genomics.lbl.gov 51 .

Garber M.E., Frank V., Kazakov A.E., Incha M.R., Nava A.A., Zhang H., Keasling J.D., Rajeev L., & Mukhopadhyay A. (2020). REC protein family expansion by the emergence of a new signaling pathway.

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