The bacteriophage population used for sequencing was obtained from a Methylomirabilis oxyfera bioreactor enrichment culture. A sample of 4 L of bioreactor effluent was collected over a period of time of 20 days and was stored at 4 °C. To concentrate the viral particles, the sample was first filtered through a 0.22 µm filter (nucleopore track-etched polycarbonate membrane filters; Whatman, Maidstone, UK) and then further concentrated using 30 kDa Vivaspin Spin Columns (GE Healthcare Life Sciences, Little Chalfont, UK), to a final volume of 3 ml. A P1 reference bacteriophage (genome sequence NC_005856.1 ) was used as a positive control for DNA extraction. The DNA extraction was performed according to the protocol published by Thurber et al. (2009) (link). Using the Qubit dsDNA HS assay kit (Life), the extracted DNA was quantified at 146 ng/ml.
Vivaspin spin columns
Manufactured by GE Healthcare
Sourced in United Kingdom
Vivaspin Spin Columns are a type of laboratory equipment used for the concentration and purification of biomolecules such as proteins, DNA, and RNA. These columns utilize a centrifugal force to separate and concentrate the target analyte from the surrounding solution.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using vivaspin spin columns
1
Bacteriophage Isolation and DNA Extraction
2
Purification of MSA/ABD-iTEP-pOVA Complex
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
MSA was incubated with ABD-iTEP-pOVA overnight at 4 °C. Then MSA/ABD-iTEP-pOVA was purified by ÄKTA fast protein liquid chromatography system (GE Healthcare) equipped with a HiPrep Sephacryl S-200 HR column (diameter: 26 mm, length: 600 mm, particle diameter: 50 µm, GE Healthcare). Samples were eluted with PBS at a flow rate of 1 mL/min. The fractions containing the MSA/ABD-iTEP-pOVA complex were combined and concentrated by Vivaspin spin columns (Molecular mass cut-off: 10,000 kDa, GE Healthcare).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!