Plant materials used in this study were previously described: PLT1pro:PLT1-YFP, PLT1pro:CFP, and PLT2pro:CFP (Galinha et al., 2007 (link)), CYCB1;1pro:GUS (Colón-Carmona et al., 1999 (link)), WOX5pro:GFP (Sarkar et al., 2007 (link)) and DII-Venus (Brunoud et al., 2012 (link)).
Arabidopsis roots from pPLT1:CFP y pPLT2:CFP transgenic plants were observed in confocal microscopy (Nikon Eclipse C1 Plus Ex/Em, 458/515).
Arabidopsis roots from TCS : GFP transgenic plants and WOX5pro:GFP were observed in confocal microscopy (Nikon Eclipse C1 Plus Ex/Em, 488/561) and quantified using Fiji software. We defined a ROI in the region that showed the most representative signal distribution. We used the same ROI (size and shape) to analyze all images of the respective experiment.
Arabidopsis root from PLT1pro:PLT1-YFP and DII-Venus were observed in confocal microscopy (Nikon Eclipse C1 Plus Ex/Em, 514/550). For DII-Venus marker we define two sections of the root, down QC is the calyptra zone, and up QC is the region above QC including QC, SCN (stem-cell niche) and a portion of meristem as indicated in Figure 4. We used the same ROI (size and shape) to analyze all images of the respective experiment.
PLT1pro:CFP, PLT1pro:PLT1-YFP and PLT2pro:CFP fluorescence was measure using Fiji software as described by Ercoli et al. (2018) (link).
Di Fino L.M., Cerrudo I., Salvatore S.R., Schopfer F.J., García-Mata C, & Laxalt A.M. (2020). Exogenous Nitro-Oleic Acid Treatment Inhibits Primary Root Growth by Reducing the Mitosis in the Meristem in Arabidopsis thaliana. Frontiers in Plant Science, 11, 1059.
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