Milliplex map human cytokine chemokine panel hcytomag 60k

Multimeric adiponectin (total, high (HMW), medium (MMW) and low (LMW) molecular weight) was measured by enzyme-linked immunosorbent assay (ELISA) following the manufacturer's instructions (Alpco Diagnostics, Salem, NH). Human leptin ELISA kits were used according to the manufacturer's instructions (R&D Systems, Minneapolis, MN). C-peptide concentrations were determined using a C-peptide ELISA (Calbiotech, Spring Valley, CA) as directed by manufacturer's protocol. The Synergy HT plate reader (Bio-Tek, Winooski, VT) was used to measure ELISA absorbencies. Serum concentrations of IL-6, IL-1α, IL-1β, IL-10, and TNF-α, were determined using a MILLIPLEX™ MAP Human Cytokine/Chemokine panel (HCYTOMAG-60K, Millipore, Billerica, MA) as per the manufacturer's protocol. The soluble receptor for IL-6 (sIL-6R) and the soluble receptor for advanced glycation end products (sRAGE) were analyzed using a MILLIPLEX™ MAP Human Soluble Cytokine Receptor panel (HSCR-32K, Millipore). MILLIPLEX™ samples were analyzed on a Bio-Plex 100 using Bio-Plex 4.1 software (Bio-Rad, Hercules, CA).

Multimeric adiponectin (total, high (HMW), medium (MMW) and low (LMW) molecular weight) was measured by ELISA following the manufacturer's instructions (Alpco Diagnostics, Salem, NH). Human leptin ELISA kits were used according to the manufacturer's instructions (R&D Systems, DY398; Minneapolis, MN). The Synergy HT plate reader (Bio-Tek, Winooski, VT) was used to measure ELISA absorbances. Serum was used to assess systemic cytokine concentrations. Serum concentrations of interleukin (IL)-6, tumor-necrosis factor-α (TNF-α), and interferon-γ-inducible protein-10 (IP-10, also known as CXCL10) were determined using a MILLIPLEX™ MAP Human Cytokine/Chemokine panel (HCYTOMAG-60K, Millipore, Billerica, MA) as per the manufacturer's protocol. Samples were analyzed on a Bio-Plex 100 using Bio-Plex 4.1 software (Bio-Rad, Hercules, CA).