After zFRAP analysis and observation, the zygotes were fixed with 4% paraformaldehyde containing 0.2% Triton X-100 for 20 min. After washing three times with PBS containing 1% BSA and 0.2% Tween 20, the zygotes were incubated with primary antibodies against H3K9me3 (ab8898; Abcam, Cambridge, MA, USA; 1:2000), Anti-H3.3 antibody (Clone 4H2D7, Cat# CE-040B, Cosmo Bio Co., LTD; 1:500), Anti-Flag M2 antibody (F1804, Merck, Darmstadt, Germany; 1:500), Anti-Hira (#39558, Active motif, Carlsbad, CA, USA; 1:500) diluted in PBS containing 1% BSA and 0.1% Triton X-100 at 4 °C overnight. After washing three times with PBS containing 1% BSA and 0.2% Tween 20, the zygotes were incubated with secondary antibodies (Alexa 568 conjugated anti-rabbit IgG, Alexa 488 conjugated mouse IgG; 1:500). The stained zygotes were mounted on PBS containing 4’,6-diamidino-2-phenylindole or Vectashield mounting medium with DAPI (Vector laboratories, INC.). The images were obtained by using confocal microscopy FV1200.
Anti hira
Manufactured by Active Motif
Sourced in Germany, United States
Anti-Hira is a primary antibody that recognizes the Hira (Histone cell cycle regulation defective homolog A) protein. Hira is a histone chaperone that plays a role in DNA replication-independent chromatin assembly.
Automatically generated - may contain errors
Lab products found in correlation
Ab8898, by Abcam (1 mentions)
Anti flag m2 antibody, by Merck Group (1 mentions)
Vectashield mounting medium with dapi, by Vector Laboratories (1 mentions)
Anti h3, by Merck Group (1 mentions)
Anti akt1, by Cell Signaling Technology (1 mentions)
Anti myogenin, by Santa Cruz Biotechnology (1 mentions)
Anti myc, by Roche (1 mentions)
Anti β catenin, by Santa Cruz Biotechnology (1 mentions)
Anti pan akt, by Cell Signaling Technology (1 mentions)
Anti asf1a, by Cell Signaling Technology (1 mentions)
Anti phospho threonine, by Abcam (1 mentions)
Anti α tubulin, by AbFrontier (1 mentions)
Anti phosphoserine, by Abcam (1 mentions)
Anti h4, by Merck Group (1 mentions)
Anti ha, by Roche (1 mentions)
Anti h3, by Abcam (1 mentions)
Anti flag, by Merck Group (1 mentions)
2 protocols using anti hira
1
Visualization of Epigenetic Markers in Zygotes
2
Antibody Characterization for Histone Regulation
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The following antibodies were used in this study: anti-HIRA (Active Motif, Carlsbad, CA, USA, WC119 and WC15), anti-Asf1a (Cell Signaling, Danvers, MA, USA, 2990), anti-Cabin1, anti-UBN1 (Abcam, Cambridge, MA, USA), anti-H3 (Abcam, ab1791), anti-H4 (Millipore, Billerica, MA, USA, 07–108), anti- H4S47p (Abcam), anti-H3.3 (Millipore, 09–838), anti-H3.1 and anti-H3.3 (provided by Dr Y Ohkawa), anti-pan-Akt, anti-Akt1, anti-Akt2, anti-p-Akt (S473) (Cell Signaling, 9271), anti-phospho-serine (Abcam, ab9332), anti-phospho-threonine (Abcam, ab9337), anti-myogenin (Santa Cruz, Santa Cruz, CA, USA, sc-576), anti-MHC (Upstate, Billerica, MA, USA, 05–716), 8WG16 (Covance, Princeton, NJ, USA, MMS-126R), anti-HA (Roche, Basel, Switzerland, 11 666 606 001), anti-Flag (Sigma-Aldrich, F3165), anti-Myc (Roche, 11 667 203 001), anti-β-catenin (Santa Cruz, sc-7963), and anti-α-tubulin (AbFrontier, Seoul, Korea, LF-PA0146). The anti-p-HIRA (Abmart, Shanghai, China) antibody was generated using the phospho-peptides RPRKD(pS)RLMPV and was affinity-purified from rabbit serum using a specific column. Antibody specificity was confirmed by checking the absence of cross-reactivity with the control peptide, RPRKDSRLMPV.
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