Ammonium tetrathiomolybdate ((NH4)2MoS4) and 1,3-Diphenylisobenzofuran (DPBF) were purchased from Heowns Biochem Technologies LLC (Tianjin, China). Hydrazine hydrate (N2H4.H2O) was obtained from Adamas Reagent Co., Ltd. (Shanghai, China). Perfuorohexane was purchased from Xianding Biological Technology co., Ltd. (Shanghai, China). Human serum albumin (HSA) and calcein-AM/PI double stain kit were received from Sigma-Aldrich (USA). Lipoic acid (LA) and 1-ethyl-3(3-(dimethylamino)-propyl) carbodiimide·hydrochloride (EDC·HCl) were purchased from Shanghai Macklin Biochemical Co., Ltd. Chloride aluminium phthalocyanine (AlPc) was obtained from Shanghai Meryer Co., Ltd. All other chemicals were analytical grade and used as received.
Calcein am pi double stain kit
Manufactured by Merck Group
Sourced in United States
The Calcein-AM/PI double stain kit is a laboratory product designed to assess cell viability and cytotoxicity. The kit contains Calcein-AM, a cell-permeant dye that is converted to a green fluorescent compound by live cells, and Propidium Iodide (PI), a dye that enters and stains the nuclei of dead or dying cells. This combination allows for the simultaneous detection of live and dead cells in a sample.
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Lab products found in correlation
2 protocols using calcein am pi double stain kit
1
Synthesis and Characterization of Photosensitizer-Loaded Nanoparticles
2
Cytotoxicity Evaluation of PTX@CAR-Exo
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The killing efficacy of PTX@CAR-Exo against MSLN-LLC cells was assessed using the Calcein-AM/PI Double Stain Kit (Sigma-Aldrich), which stains live and dead cells. Briefly, 1 × 105 MSLN-LLC cells per well were seeded in 12-well plates. Then, 100 μL of PBS, 100 μg of T-Exos, 100 μg of CAR-Exos, 100 μL of PBS containing 10 μg of PTX, 100 μg of T-Exos containing 10 μg of PTX, and 100 μg of CAR-Exos containing 10 μg of PTX were added to the Petri dishes. After incubation for 24 h, the live and dead cells stained with Calcein-AM/PI were observed under an inverted fluorescence microscope (Olympus). Then, quantification was performed using ImageJ software.
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