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Rabbit anti cd68 monoclonal antibody

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Rabbit anti-CD68 monoclonal antibody is a laboratory reagent used for the detection and analysis of the CD68 protein. CD68 is a glycoprotein expressed by cells of the monocyte-macrophage lineage and is commonly used as a marker for macrophages. This antibody can be used in various immunoassay techniques to identify and study macrophages in biological samples.

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Lab products found in correlation

Endogenous peroxidase blocking solution, by Vector Laboratories (2 mentions) Anti rabbit igg secondary antibody, by Vector Laboratories (2 mentions) Rat anti f4 80 monoclonal antibody, by Abcam (1 mentions) Rabbit anti gapdh monoclonal antibody, by Abcam (1 mentions) 1 4 diazabicyclo 2.2.2 octane, by Merck Group (1 mentions) Rabbit anti β actin monoclonal antibody, by Cell Signaling Technology (1 mentions) Rabbit anti cd163 monoclonal antibody, by Abcam (1 mentions) Rabbit monoclonal anti notch1 antibody, by Cell Signaling Technology (1 mentions) Quercetin, by Merck Group (1 mentions) Carbon tetrachloride, by Merck Group (1 mentions) Dm6000b microscope, by Leica (1 mentions)

Spelling variants of this product

Anti-CD68 (167 citations) Anti-CD68 antibody (56 citations) Rabbit anti-CD68 (19 citations) CD68 antibody (17 citations) Rabbit anti-CD68 antibody (12 citations) Rabbit anti- (5 citations)

4 protocols using rabbit anti cd68 monoclonal antibody

1

Investigating Quercetin and CCl4-Induced Liver Injury

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Carbon tetrachloride (CCl4), quercetin, dimethyl sulfoxide (DMSO), olive oil, 1,4-diazabicyclo[2.2.2]octane (DABCO), and lipopolysaccharide (LPS; from Escherichia coli 0727: B8) were purchased from Sigma Chemical, Co., Ltd. (St. Louis, MO, United States). For in vivo and in vitro experiments, quercetin was diluted immediately in DMSO solution before administration.
Antibodies used in this study comprised: mouse anti-desmin monoclonal antibody (DakoCytomation, Glostrup, Denmark); rabbit anti-collagen III polyclonal antibody, rabbit anti-collagen IV polyclonal antibody, rabbit anti-CD68 monoclonal antibody, rat anti-F4/80 monoclonal antibody, mouse anti-CD11c polyclonal antibody, mouse anti-IRF5 monoclonal antibody, rabbit anti-Ym-1 monoclonal antibody, rabbit anti-CD163 monoclonal antibody, and rabbit anti-GAPDH monoclonal antibody (Abcam, Cambridge, MA, United States); rabbit anti-IL12a monoclonal antibody, rabbit anti-Notch1 monoclonal antibody, and rabbit anti-β-actin monoclonal antibody (Cell Signaling Technology, Boston, MA, United States).
Li X., Jin Q., Yao Q., Xu B., Li L., Zhang S, & Tu C. (2018). The Flavonoid Quercetin Ameliorates Liver Inflammation and Fibrosis by Regulating Hepatic Macrophages Activation and Polarization in Mice. Frontiers in Pharmacology, 9, 72.
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2

Quantitative Immunohistochemistry for Macrophages and Neutrophils

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For IHC, endogenous peroxidase activity of paraffin-embedded sections was blocked with 0.3% hydrogen peroxide in methanol. Sections, each separated by 3–5 μm, were washed with PBS and incubated with rabbit anti-CD68 monoclonal antibody (Abcam, 1:500) to stain macrophages and rabbit anti-CD11b monoclonal antibody (Abcam, 1:500) to stain neutrophils at 4°C overnight. Specimens were then treated with a peroxidase conjugated goat anti-mouse secondary antibody (ZSGB Biotech, CA) and diaminobenzene substrate (ZSGB Biotech). Images were taken using a Leica DM 6000B microscope (Leica, Germany). The semi-quantitative analysis was performed by ImageJ analysis software.
Meng Z., Li Z., Zhang E., Zhang L., Liu Q, & Wu Y. (2021). Sevelamer Attenuates Bioprosthetic Heart Valve Calcification. Frontiers in Cardiovascular Medicine, 8, 740038.
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3

Immunohistochemical Evaluation of CD68 in Mouse Brains

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Mouse brains were collected and fixed in 2% paraformaldehyde. After 72 h, the tissues were washed with water and placed in 70% ethanol. Brains were embedded in paraffin and sectioned. Briefly, sections were deparaffinized and rehydrated. Antigen retrieval was performed by boiling for 30 min. Slides were treated with an endogenous peroxidase blocking solution (Vector, USA), followed by another block with 3% bovine serum albumin. Anti-CD68 rabbit monoclonal antibody (Abcam, USA, 1:500) was incubated at 4 C overnight in a humidified chamber. Anti-rabbit IgG secondary antibody was added (Vector), followed by diaminobenzidine substrate for 5 min and counterstained with hematoxylin. All slides were read blinded by a single reader.
Sun B., Kitchen S., Tang N., Garza A., Jacob S, & Pulliam L. (2022). Engineered induced-pluripotent stem cell derived monocyte extracellular vesicles alter inflammation in HIV humanized mice. Extracellular vesicles and circulating nucleic acids, 3(2), 118-132.
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4

Immunohistochemical Evaluation of CD68 in Mouse Brains

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Mouse brains were collected and fixed in 2% paraformaldehyde. After 72 h, the tissues were washed with water and placed in 70% ethanol. Brains were embedded in paraffin and sectioned. Briefly, sections were deparaffinized and rehydrated. Antigen retrieval was performed by boiling for 30 min. Slides were treated with an endogenous peroxidase blocking solution (Vector, USA), followed by another block with 3% bovine serum albumin. Anti-CD68 rabbit monoclonal antibody (Abcam, USA, 1:500) was incubated at 4 C overnight in a humidified chamber. Anti-rabbit IgG secondary antibody was added (Vector), followed by diaminobenzidine substrate for 5 min and counterstained with hematoxylin. All slides were read blinded by a single reader.
Sun B., Kitchen S., Tang N., Garza A., Jacob S, & Pulliam L. (2022). Engineered induced-pluripotent stem cell derived monocyte extracellular vesicles alter inflammation in HIV humanized mice. Extracellular vesicles and circulating nucleic acids, 3(2), 118-132.
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