IPSC lines were allowed to differentiate by EB formation as described (Toombs et al., 2020 ). TaqMan qRT-PCR was carried out using the hPSC Scorecard 384w Fast plate (Life Technologies) and QuantStudio 12 k Flex following the manufacturer’s protocol. Gene expression data was analyzed using web-based hPSC Scorecard Analysis Software (ThermoFisher) (Fig. 1D ).
Hpsc scorecard 384w fast plate
Manufactured by Thermo Fisher Scientific
The HPSC Scorecard 384w Fast plate is a high-performance 384-well microplate designed for fast, efficient, and accurate sample processing in laboratory applications. The plate features a compact footprint and is engineered to provide consistent results across multiple samples.
Automatically generated - may contain errors
Lab products found in correlation
Quantstudio 12k flex, by Thermo Fisher Scientific (2 mentions)
Hpsc scorecard analysis software, by Thermo Fisher Scientific (2 mentions)
Taqman hpsc scorecard panel, by Thermo Fisher Scientific (1 mentions)
Magmax mirvana total rna isolation kit, by Thermo Fisher Scientific (1 mentions)
High capacity cdna reverse transcription kit, by Thermo Fisher Scientific (1 mentions)
2 protocols using hpsc scorecard 384w fast plate
1
Pluripotency Profiling of iPSCs
2
Pluripotency and Differentiation Assessment of iPSC Lines
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Applied Biosystems TaqMan®hPSC Scorecard™ Panel (Thermo Fisher Scientific) was used as an additional technique to assess pluripotency and tri-lineage differentiation potential of iPSC lines using real-time qPCR assays (Fig. 1 E, Supplementary Figs. 2–24E ). Total RNA from undifferentiated and EB differentiated iPSC lines was isolated using MagMAX™ mirVana™ Total RNA Isolation Kit (A27828), and 1 μg of RNA was used to make cDNA using the High Capacity cDNA Reverse Transcription Kit (4368813), both from Applied Biosystems. TaqMan qRT-PCR was carried out using the hPSC Scorecard 384w Fast plate (Life technologies, A15870) and QuantStudio 12 k Flex, following manufacturer protocol. We analysed the gene expression data from the TaqMan®hPSC Scorecard™ Panel using the web-based hPSC Scorecard™ Analysis Software (Thermo Fisher Scientific).
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