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Apc conjugated cd235a gpa

Manufactured by BD

APC-conjugated CD235a (GPA) is a fluorescently-labeled antibody that binds to the CD235a (Glycophorin A) cell surface antigen. CD235a is primarily expressed on red blood cells and can be used to identify erythroid lineage cells.

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2 protocols using apc conjugated cd235a gpa

1

Multiparameter Flow Cytometry Analysis

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APC-conjugated CD235a (GPA), PE-conjugated-CD34, FITC-conjugated-CD36 and cell viability marker 7-AAD were purchased from BD Biosciences, PE-conjugated α4 integrin from Miltenyi Biotec, PE-Cyanine7-conjugated-IL-3R (CD123) from eBioscience, and a mouse mAb against human Band3 was used as previously described[18 (link)].
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2

Monoclonal Antibodies for Hematology Analysis

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Mouse monoclonal antibodies against human band 3 and Kell were generated in our laboratory [3] (link), [20] (link), [21] (link). Commercial antibodies included BV421-conjugated CD45 (Catalog No. 563879, BD Biosciences, Franklin Lakes, NJ), PE-conjugated CD235a/GPA (Catalog No. 555570, BD Biosciences), APC-conjugated CD235a/GPA (Catalog No. 551336, BD Biosciences), PE-Cy7-conjugated CD123 (Catalog No. 25-1239-42, Invitrogen, Carlsbad, CA), APC-conjugated CD49d (Catalog No. 304307, BioLegend, San Diego, CA), PE-conjugated CD34 (Catalog No. 555822, BD Biosciences), APC-conjugated CD36 (Catalog No. 550956, BD Biosciences), FITC-conjugated CD36 (Catalog No. 555454, BD Biosciences), PE-conjugated CD49e (Catalog No. 103805, BioLegend), FITC-conjugated CD47 (Catalog No. 556045, BD Biosciences), PE-conjugated CD71 (Catalog No. 555537, BD Biosciences), AF647-conjugated CD147 (Catalog No. 562551, BD Biosciences), and Hoechst 33342 (Catalog No. 561908, BD Biosciences).
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