All fluorescent strains were treated with appropriate RNAi condition at 24°C for 24 h before imaging. 10 µl of anesthetic (0.1% tricane and 0.01% tetramisole in 1X M9 buffer) was added to a 3% agar pad on a slide and 10–15 live worms were transferred to the drop of anesthetic. A glass coverslip was slowly lowered to cover the samples and the coverslip edges were sealed with nail polish and allowed to dry before imaging. Images were obtained on a Nikon Ti-E-PFS inverted spinning-disk confocal microscope using a 60X 1.4NA Plan Apo Lambda objective. The microscope consists of a Yokowaga CSU-X1 spinning disk unit, a self-contained 4-line laser module (excitation at 405, 488, 561, and 640 nm), and an Andor iXon 897 EMCDD camera. Fluorescence intensities were quantified and image editing done using NIS-elements software.
4 line laser module
Manufactured by Oxford Instruments
The 4-line laser module is a compact and powerful device that generates four distinct laser beams. It produces stable and aligned laser lines that can be used for various applications requiring precise optical alignment or positioning.
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Lab products found in correlation
2 protocols using 4 line laser module
1
Fluorescent Worm Imaging Procedure
2
Live Worm Imaging on Spinning-Disk Confocal
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All fluorescent strains were treated with appropriate RNAi condition at 24°C for 24hrs before imaging. 10µl of anesthetic (0.1% tricane and 0.01% tetramisole in 1X M9 buffer) was added to a 3% agar pad on a slide and 10-15 live worms were transferred to the drop of anesthetic. A glass coverslip was slowly lowered to cover the samples and the coverslip edges were sealed with nail polish and allowed to dry before imaging. Images were obtained on a Nikon Ti-E-PFS inverted spinning-disk confocal microscope using a 60x 1.4NA Plan Apo Lambda objective. The microscope consists of a Yokowaga CSU-X1 spinning disk unit, a self-contained 4-line laser module (excitation at 405, 488, 561, and 640nm), and an Andor iXon 897 EMCDD camera. Fluorescence intensities were quantified and image editing done using NIS-elements software.
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