Wash buffer

Reagents were prepared prior to RNA extraction according to manufacturer’s instructions. The protocol was conducted in a Class I MSC in a CL-3 lab. From a swab tube, 140 μl were transferred to a Zymo-Spin I-96 Plate (Zymo Research). 7 μl of Proteinase K/PK buffer and 105 μl of Lysis buffer was added to each sample, mixed and incubated at room temperature for 15 minutes. Following incubation, 143 μl of Bind1/Isopropanol was added to each sample, mixed, and the samples were left to incubate at room temperature for 5 min. The Zymo-Spin I-96 Plate was placed on ZR-96 MagStand (Zymo Research), and the magnetic beads left to form a pellet. The supernatant was removed, and the magnetic beads washed three times, first, with 280 μl of Wash buffer (Beckman Coulter), followed by two washes with 70% ethanol. Following the wash steps, RNA was eluted from the columns with 80 μl RNase-free water (Ambion).

Reagents were prepared prior to RNA extraction according to manufacturer’s instructions. The protocol was conducted in a Class I MSC in a CL-3 lab. From a swab tube, 140 μl were transferred to a Zymo-Spin I-96 Plate (Zymo Research). 7 μl of Proteinase K/PK buffer and 105 μl of Lysis buffer was added to each sample, mixed and incubated at room temperature for 15 minutes. Following incubation, 143 μl of Bind1/Isopropanol was added to each sample, mixed, and the samples were left to incubate at room temperature for 5 min. The Zymo-Spin I-96 Plate was placed on ZR-96 MagStand (Zymo Research), and the magnetic beads left to form a pellet. The supernatant was removed, and the magnetic beads washed three times, first, with 280 μl of Wash buffer (Beckman Coulter), followed by two washes with 70% ethanol. Following the wash steps, RNA was eluted from the columns with 80 μl RNase-free water (Ambion).