Hcc lm3

Human hepatocellular carcinoma cells MHCC-97H and HCC-LM3, and human umbilical vein endothelial cells (HUVEC) were purchased from Cell Bank, Chinese Academy of Sciences (Shanghai, China). HCC-LM3 cells were cultured in DMEM, MHCC-97H and HUVEC cells were cultured in RPMI-1640, supplemented with 10% Fetal Bovine Serum (FBS, Wisent, Inc., CAN, Quebec, QC, Canada) and 100 U/ml of streptomycin and penicillin (GIBCO, Thermo Fisher Scientific Inc., Chino, CA, USA) at 37 °C with 5% CO₂.
The anchorage-independent growth of cells was performed in six-well plate coated with poly-2-hydroxyethylmethacrylate (poly-HEMA, Sigma-Aldrich, St. Louis, MO, USA). poly-HEMA powder was dissolved to 12 mg/mL in 95% ethanol.

The LO₂ (human embryonic liver) cell line and the Huh7 (human HCC) cell line were purchased from the Cell Bank of Xiangya Central Laboratory, Central South University. Three liver cancer cell lines, HCCLM3, MHCC‐97 L, and MHCC‐97H, were gifts from the Liver Cancer Institute, Zhongshan Hospital, Fudan University. SMMC‐7721, HepG2, Bel‐7402, Hep3B, PLC, and Li‐7 cell lines were obtained from the cell bank of the Chinese Academy of Sciences. HCCLM3, MHCC‐97 L and MHCC‐97H, HepG2, Hep3B, SMMC‐7721, PLC, Huh7, and Li‐7 cells were cultured in complete DMEM (Wisent, Inc.). LO2 and Bel‐7402 cells were cultured in complete RPMI‐1640 medium (Wisent, Inc.). All cells were supplemented with 10% fetal bovine serum (Wisent, Inc.) at 37°C in a humidified incubator with 5% CO₂.