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2 protocols using antibodies against cytochrome c

1

Molecular Mechanisms of ER Stress Response

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Antibodies against Bip, p-PERK, PERK, p-IRE1, IRE1, ATF6, Chop and Caspase-3 were obtained from Cell Signaling Technology, Inc. Antibodies against cytochrome c, cytochrome c oxidase IV (COX IV) and GAPDH were purchased from Abcam. Phosphorylated (p)-Bcl-2 (Ser70) rabbit mAb, p-JNK (Thr183/Tyr185) rabbit mAb and JNK rabbit mAb were obtained from Cell Signaling Technology, Inc. LC3 rabbit mAb was purchased from Santa Cruz Biotechnology, Inc. The MDA Assay kit (TBA method) and Hydrogen Peroxide Assay kit were obtained from Nanjing Jiancheng Bioengineering Institute. An In Situ Cell Death Detection kit was obtained from Roche Applied Science. Dexamethasone (DEX) was purchased from MilliporeSigma. Tat-scramble (LPS VFG DVG APS RLP EVS LSP PRR RQR RKK RG-NH2) and Tat-SabKIM1 (GFE SLS VPS PLD LSG PRV VAP PRR RQR RKK RG-NH2) were purchased from NeoPeptide. TRIzol was purchased from Thermo Fisher Scientific, Inc.
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2

Apoptosis Signaling Pathway Analysis

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Dulbecco's modified Eagle's medium (DMEM) and fetal bovine serum (FBS) were purchased from Gibco (Carlsbad, CA). Trypsin and collagenase were purchased from Sigma-Aldrich (MO, USA). Antibodies against Bax, Bcl-2, cleaved-caspase-3, and phospho-p38 MAPK were purchased from Cell Signaling Technology (MA, USA). Antibodies against cytochrome c was purchased from Abcam (MA, USA). Antibodies against UCP2, Cox-IV, and GAPDH were purchased from Proteintech (IL, USA).
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