RAW264.7 murine macrophages were cultured in a 96-well plate (1×104cells/well) for 24 h. Medium with a final concentration of 0, 1, 5, 10, 20, 60, 100, 200, 300 μM compounds was added, with each concentration repeated across 6 wells (n = 6). The negative control comprised cells treated with 0.1% of dimethyl sulphoxide (DMSO), diluted in complete DMEM, and incubated at 37°C for 24 h. After treatment for 24 h, 10 μL of Cell Counting Kit (CCK-8) assay solution (Beyotime, Beijing, China) was added to each well, and cells were incubated for 1 h at 37°C and 5% CO2. The absorbance at 450 nm was measured using a microplate reader (SPARK 10M, Thermo, Tecan). Cell viability was calculated as absorbance/absorbance of control ×100%.
Cell counting kit cck 8 assay solution
Manufactured by Beyotime
Sourced in China
The Cell Counting Kit (CCK-8) assay solution is a colorimetric assay for the determination of cell viability and proliferation. The kit utilizes the highly water-soluble tetrazolium salt WST-8, which is reduced by dehydrogenases in living cells to produce a yellow-colored formazan dye. The amount of the formazan dye is directly proportional to the number of living cells.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using cell counting kit cck 8 assay solution
1
Cytotoxicity Assay of Compounds in RAW264.7 Cells
2
Evaluating LHZZ's Cytotoxicity on IH-CRA Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
IH-CRA cells in the logarithmic phase were seeded at 1 × 104cells/well in 96-well plates. After incubation for 24 h, IH-CRA cells were exposed to LHZZ (0, 25, 50, 75 and 100 μg/ml). After treatment for 48 h and 72h, 20 ml of Cell Counting Kit (CCK-8) assay solution (Beyotime, Shanghai, China) was added to each well, and cells were incubated for 4 h at 37°C and 5% CO2. The absorbance at 450 nm was measured by a microplate reader (FLUOstar Omega, LABTECH, Offenburg, Germany). Cell survival was calculated as: absorbance/absorbance of control × 100%. The Graphpad prism 7.0 software was used to analyze and plot the data.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!