Total protein was harvested from cultured HCC cells. According to the manufacturer’s instructions, coimmunoprecipitation (Co-IP) was conducted, and an anti-Bcl-2 antibody (1:500, ABclonal, China), an anti-Beclin-1 antibody (1:500, Proteintech, USA), and protein A/G agarose (MedChemExpress, USA) were used. Immunoprecipitated proteins were collected and then used for Western blot analysis to evaluate the interaction between Beclin-1 and Bcl-2.
Protein a g agarose
Manufactured by MedChemExpress
Sourced in United States
Protein A/G agarose is a chromatography resin composed of recombinant Protein A and Protein G covalently coupled to agarose beads. It is designed for the purification of immunoglobulins and other Fc-containing proteins from a variety of sample sources.
Automatically generated - may contain errors
Lab products found in correlation
Anti beclin1 antibody, by Proteintech (1 mentions)
Anti bcl 2 antibody, by ABclonal (1 mentions)
Anti phosphotyrosine, by Merck Group (1 mentions)
Anti siah1, by Merck Group (1 mentions)
Verteporfin, by MedChemExpress (1 mentions)
Mg132, by Targetmol (1 mentions)
Anti β trcp, by Cell Signaling Technology (1 mentions)
Anti p yap ser127, by Cell Signaling Technology (1 mentions)
Anti lats1, by Cell Signaling Technology (1 mentions)
Anti p lats1 ser909, by Cell Signaling Technology (1 mentions)
Anti ha 3724, by Cell Signaling Technology (1 mentions)
Polybrene, by Merck Group (1 mentions)
Polyjet, by SignaGen (1 mentions)
Cycloheximide (chx), by Targetmol (1 mentions)
Puromycin, by MedChemExpress (1 mentions)
Anti β actin, by ABclonal (1 mentions)
2 protocols using protein a g agarose
1
Bcl-2 and Beclin-1 Interaction Assay
2
Protein Analysis using Diverse Antibodies
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Primary antibodies used were as follows: Anti-HA (#3724), anti-p-YAPSer127 (#13008), anti-LATS1 (#3477), anti-p-LATS1Ser909 (#9157), and anti-β-Trcp (#4394) were from Cell Signaling Technology. Anti-YAP (WH0010413M1), anti-Flag (F1804), anti-PCNA (05-347), and anti-Siah1 (AV38212) were from Sigma. Anti-p-YAPTyr357 (ab62751), anti-YAP (ab52771), and anti-SKP1 (ab76502) were from abcam. Anti-Phosphotyrosine (#05-321) was from Millipore. Anti-FRK (sc-166478), anti-CYR61 (sc-374129), and anti-CTGF (sc-101586) were from Santa Cruz Biotechnology. Anti-LATS2 (A16249), anti-p-LATS1T1079/LATS2T1041 (AP0912), anti-β-actin (AC026), and anti-Siah1 (A2494) were from ABclonal. Cycloheximide (CHX) and MG132 were from TargetMol. Puromycin, Protein A/G agarose and verteporfin (VP) were from MedChemExpress. PolyJet was from Signagen. Polybrene was from Sigma.
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