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Acetyl histone h4 lys8

Manufactured by Cell Signaling Technology
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Acetyl-histone H4 (Lys8) is a lab equipment product that serves as an antibody targeting the acetylation of lysine 8 on histone H4. This modification is associated with chromatin remodeling and transcriptional regulation.

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Lab products found in correlation

Acetyl histone h3 lys9, by Cell Signaling Technology (2 mentions) Anti pd l1, by Cell Signaling Technology (1 mentions) Anti gapdh, by Cell Signaling Technology (1 mentions) Anti β actin, by Proteintech (1 mentions) Antibodies to actin, by Merck Group (1 mentions) Phospho foxo1 ser256, by Cell Signaling Technology (1 mentions) Phospho erk1 2, by Cell Signaling Technology (1 mentions) Phospho 4e bp1 thr37 46, by Cell Signaling Technology (1 mentions) Phospho akt thr308, by Cell Signaling Technology (1 mentions) Foxo1, by Cell Signaling Technology (1 mentions) Phospho akt ser473, by Cell Signaling Technology (1 mentions) Vegfr2, by Cell Signaling Technology (1 mentions) Acetyl histone h4, by Cell Signaling Technology (1 mentions) Acetyl histone h3 lys18, by Cell Signaling Technology (1 mentions) Pgc 1a, by Cell Signaling Technology (1 mentions) Histone h2b, by Cell Signaling Technology (1 mentions) Acetyl histone h4 lys5, by Cell Signaling Technology (1 mentions) Histone h2a, by Cell Signaling Technology (1 mentions) Tead4, by Abcam (1 mentions) Acetyl histone h3 lys56, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

Acetyl-histone H4 Acetyl-H4 Histone H4

3 protocols using acetyl histone h4 lys8

1

Antibody Validation for Protein Analysis

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The antibodies used in this study included acetyl-histone H3 (Lys9) (Cat. 9649T, Cell Signaling Technology, Danvers, MA, USA), acetyl-histone H4 (Lys8) (Cat. 594T, Cell Signaling Technology, Danvers, MA, USA), anti-PD-L1 (Cat. Ab238697, abcam, Cambridge, MA, USA), anti-GAPDH (Cat. 2118S, Cell Signaling Technology, Danvers, MA, USA), anti-β-actin (Cat. 20536-1-AP, Proteintech, Rosemont, IL, USA), and anti-nectin-1 (Cat. 37-5900, Invitrogen, Waltham, MA, USA).
Wu Y., Chen X., Wang L., Zhou X., Liu Y., Ji D., Ren P., Zhou G.G, & Zhao J. (2022). Histone Deacetylase Inhibitor Panobinostat Benefits the Therapeutic Efficacy of Oncolytic Herpes Simplex Virus Combined with PD-1/PD-L1 Blocking in Glioma and Squamous Cell Carcinoma Models. Viruses, 14(12), 2796.
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2

Quantitative Immunoblot Analysis of Cell Signaling

Cited 1 time
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Quantitative immunoblot analyses were conducted using standard procedures [16 (link)]. Antibodies directed to ERK1/2 (#9102), phospho-ERK1/2 (#9101), VEGFR2 (#9698), phospho-VEGFR2 Tyr1115 (#3770), acetyl-histone H4 Lys8 (#2594), phospho-AKT Thr308 (#4056), phospho-AKT Ser473 (#3787), AKT (#4691), phospho-FOXO1 Ser256 (#9461), FOXO1 (#2880), phospho-4EBP1 Thr37/46 (#2855), phospho-S6K Ser235/236 (#4858) were purchased from Cell Signaling Technology and used at dilutions of 1:1000. Antibodies to RET (# ab134100) and phospho-RET Tyr 1062 (#ab51103) were purchased from Abcam and used at dilutions of 1:10,000 and 1:2000 respectively. Antibodies to Actin (#A2228) were from Sigma-Aldrich and used at dilution 1:10,000.
Pozo K., Zahler S., Ishimatsu K., Carter A.M., Telange R., Tan C., Wang S., Pfragner R., Fujimoto J., Grubbs E.G., Takahashi M., Oltmann S.C, & Bibb J.A. (2018). Preclinical characterization of tyrosine kinase inhibitor-based targeted therapies for neuroendocrine thyroid cancer. Oncotarget, 9(102), 37662-37675.
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3

Comprehensive Protein Analysis Protocol

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TEAD4 (#ab58310), pan-OXPHOS (#ab110413), and Ki67 (#ab15580) were purchased from Abcam. p-mTOR (T2446) (#09-345) and acetyl-histone H3 (#06-599) were purchased from Millipore. Phosphor-PGC1a (#AF6650) was purchased from R&D. mTOR (#2938), p-mTOR (S2448) (#5536), YAP (#14074), p-YAP (S127) (#13008), p-YAP(S397) (#13619), p38 (#8690), p-p38 (T180/182) (#4511), acetyl-histone H3 (Lys9) (#9649), acetyl-histone H3 (Lys14) (#7627), acetyl-histone H3 (Lys18) (#13998), acetyl-histone H3 (Lys27) (#8173), acetyl-histone H3 (Lys56) (#4243), histone H3 (#4499), acetyl-histone H2A (Lys5) (#2579), acetyl-histone H2B (Lys5) (#12799), histone H2A (#12349), histone H2B (#12364), acetyl-histone H4 (Lys5) (#8647), acetyl-histone H4 (Lys8) (#2594), acetyl-histone H4 (Lys12) (#13944), acetyl-histone H4 (#2935), PGC-1a (#2178), p21 (#2974), and p27 (#83630) were purchased form Cell Signaling. All antibodies were used at a dilution of 1:1000 for immunoblotting and 1:250 for immunofluorescent staining.
Chen C.L., Hsu S.C., Chung T.Y., Chu C.Y., Wang H.J., Hsiao P.W., Yeh S.D., Ann D.K., Yen Y, & Kung H.J. (2021). Arginine is an epigenetic regulator targeting TEAD4 to modulate OXPHOS in prostate cancer cells. Nature Communications, 12, 2398.
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