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Ab218164

Manufactured by Abcam
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Ab218164 is a lab equipment product from Abcam. It is a device used for scientific research purposes.

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Lab products found in correlation

Benchmark ultra, by Roche (1 mentions) Ab53066, by Abcam (1 mentions) Ab28244, by Abcam (1 mentions) Ab207178, by Abcam (1 mentions) Ab227703, by Abcam (1 mentions) Mab3715, by R&D Systems (1 mentions) Ir700, by LI COR (1 mentions) Af3715, by R&D Systems (1 mentions)

2 protocols using ab218164

1

Immunohistochemical Analysis of FAP and SSTR2 in NPC

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Tissue microarrays of human NPC (HNasN110su01) were purchased from Shanghai Outdo Biotech Co. The Ethics Committee of the Shanghai Outdo Biotech Co. approved the study. Continuous sections of tumor tissue microarrays were used to ensure consistency in verifying different biomarkers in immunohistochemistry experiments. For immunohistochemistry analysis of paraffin-embedded areas, the BenchMark ULTRA (Ventana Medical Systems) automated slide stainer was used to stain cells with the anti-FAP antibody (ab218164; Abcam) or anti-SSTR2 antibody (ZA-0587; ZAGB-BIO) according to the manufacturer’s recommendation. The sections were visualized, and images were captured using a Leica microscope. FAP and SSTR2 expression was semiquantitatively evaluated using the H-score method (20 (link)). Negative expression was defined as an H-score of less than 10.
Zhao L., Pang Y., Fang J., Chen J., Zhou Y., Sun L., Wu H., Guo Z., Lin Q, & Chen H. (2024). Design, Preclinical Evaluation, and Clinical Translation of 68Ga-FAPI-LM3, a Heterobivalent Molecule for PET Imaging of Nasopharyngeal Carcinoma. Journal of Nuclear Medicine, 65(3), 394-401.
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2

FAP-α Antibody Sourcing Protocol

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Water soluble phthalocyanine dye IR700 was obtained from Li‐Cor Bioscience. Anti‐FAP‐α polyclonal sheep Ab, AF3715, monoclonal mouse Ab MAB3715, and monoclonal rat Ab MAB9729 were purchased from R&D Systems. Anti‐FAP‐α monoclonal mouse Ab BMS168 was purchased from eBioscience. Anti‐FAP‐α Abs, ab137549, ab218164, ab28244, ab53066, ab207178, and ab227703, were purchased from Abcam. Anti‐FAP‐α polyclonal rabbit Ab PA5‐51057 and sheep IgG isotype control (Cat. No. 31243) were purchased from Thermo Fisher Scientific.
Jin J., Barnett J.D., Krishnamachary B., Mironchik Y., Luo C.K., Kobayashi H, & Bhujwalla Z.M. (2022). Evaluating near‐infrared photoimmunotherapy for targeting fibroblast activation protein‐α expressing cells in vitro and in vivo. Cancer Science, 114(1), 236-246.
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