Phenylmethylsulfonyl fluoride pmsf

Octyl-Sepharose Fast Flow, Sephadex G-25, SP-Sepharose High Performance, and Source 15PHE were purchased from GE Life Sciences (Pittsburgh, PA, USA). Fibrinogen, thrombin (bovine and human source), immunoglobulin G, and human serum albumin (Shanghai Jingke Biological Co., Ltd., Shanghai, China). Ammonium sulfate, agarose, a low-molecular-weight protein standard kit for SDS-PAGE, and sodium chloride (Sangon Biotech Co., Ltd., Shanghai, China). Soybean trypsin inhibitor (SBTI), N-α-tosyl-L-phenylalanine chloromethyl ketone (TPCK), phenylmethylsulfonyl fluoride (PMSF), aprotinin, pepsin, and pepstatin (Sangon Biotech Co., Ltd., Shanghai, China). Coomassie Brilliant Blue R-250 (Sangon Biotech Co., Ltd., Shanghai, China). KCl, NaCl, NaHCO₃, CaCl₂, and HCl (Kemi Ou Chemical Reagent Co., Ltd., Tianjin, China).

Triptolide was purchased from Sichuan Weikeqi-Biotech Company (Chengdu, China); Cycloheximide (CHX) was purchased from Dalian Meilun Biotechnology (Dalian, China); Probenecid was purchased from Shanghai Aladdin Bio-Chem Technology Co., Ltd. (Shanghai, China); A23187 was purchased from BioVision, Inc. (Milpitas, CA, USA), BAPTA-AM was purchased from Selleck Chemicals (Houston, TX, USA); ALLM was purchased from ApexBio Technology (Houston, TX, USA); FLUO-3 AM was purchased from KeyGEN Biotech (Nangjing, China); Phenylmethylsulfonyl fluoride (PMSF) was purchased Sangon Biotech Co., Ltd. (Shanghai, China).