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Silrp1 or sictrl duplexes

Manufactured by Merck Group
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SiLRP1 or siCtrl duplexes are laboratory products that can be used in various research applications. They are short, double-stranded RNA molecules designed for gene silencing experiments. The core function of these duplexes is to serve as tools for targeted gene knockdown, allowing researchers to study the effects of reduced expression of specific genes. However, a more detailed description cannot be provided while maintaining an unbiased and purely factual approach without extrapolation on the intended use.

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Lab products found in correlation

Rnaimax, by Merck Group (2 mentions)

2 protocols using silrp1 or sictrl duplexes

1

Knockdown of LRP1 in HCT116 cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
HCT116 cells were plated at 40% confluency in 6-well plates, after which the cells were transfected with siLRP1 or siCtrl duplexes (40 μM; Sigma, St.Louis, MO) using RNAiMAX according to the protocol recommended by the manufacturer. The transfection was conducted for either 72 hr or for the indicated amount of time before the cells were collected and analyzed. The following small interfering RNA (siRNA) constructs were used (sense strand displayed): siCtrl, 5ˊ-CAG UCGCGUUUGCGACUGG[dT][dT]-3ˊ; siLRP1–1, 5ˊ-GACUUGCAGCCCCAAG CAGUU[dT][dT]-3ˊ; siLRP1–2, 5ˊ-GCAGUUUGCCUGCAGAGAUUU[dT][dT]-3ˊ.
Leslie P.L., Franklin D.A., Liu Y, & Zhang Y. (2018). p53 Regulates the Expression of LRP1 and Apoptosis through a Stress Intensity-Dependent MicroRNA Feedback Loop. Cell reports, 24(6), 1484-1495.
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2

Knockdown of LRP1 in HCT116 cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
HCT116 cells were plated at 40% confluency in 6-well plates, after which the cells were transfected with siLRP1 or siCtrl duplexes (40 μM; Sigma, St.Louis, MO) using RNAiMAX according to the protocol recommended by the manufacturer. The transfection was conducted for either 72 hr or for the indicated amount of time before the cells were collected and analyzed. The following small interfering RNA (siRNA) constructs were used (sense strand displayed): siCtrl, 5ˊ-CAG UCGCGUUUGCGACUGG[dT][dT]-3ˊ; siLRP1–1, 5ˊ-GACUUGCAGCCCCAAG CAGUU[dT][dT]-3ˊ; siLRP1–2, 5ˊ-GCAGUUUGCCUGCAGAGAUUU[dT][dT]-3ˊ.
Leslie P.L., Franklin D.A., Liu Y, & Zhang Y. (2018). p53 Regulates the Expression of LRP1 and Apoptosis through a Stress Intensity-Dependent MicroRNA Feedback Loop. Cell reports, 24(6), 1484-1495.
+ Open protocol
+ Expand

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