Mibolerone mib

The following primary antibodies were obtained from Santa Cruz Biotechnology Inc., USA; GR(H-300): sc-8992, PR(C-20) (which detects PRA and B isoforms): sc-539, AR(441): sc-7305, GAPDH(0411): sc-47724, MR(MCR, H300): sc-11412, ERα(MC-20): sc-542. The flotillin-1 (610820) antibody was purchased from BD Transduction Laboratories (USA). The following secondary antibodies were obtained from Santa Cruz Biotechnology Inc., USA; anti-mouse: sc-2005, anti-goat: sc-2350 (used as IgG for the ChIP assay) and anti-rabbit: sc-2313. The ligands dexamethasone (DEX), MPA, P4, NET-A, NET, aldosterone (ALD) and mibolerone (MIB) were obtained from Sigma-Aldrich (South Africa). Human tumour necrosis factor α (TNFα) was obtained from Celtic Diagnostics (South Africa). Protease inhibitor cocktail tablets (EDTA-free) (cat #04693159001) were obtained from Roche (South Africa). Cycloheximide (CHX) was purchased from Sigma-Aldrich (South Africa).

The primary antibodies for NCAPD3 and PCLAF were obtained from Santa Cruz Biotechnology (CA, USA). Other primary antibodies for STAT3, AR, MYC, EZH2, AGO2, Ki67, β-actin and the secondary antibodies, including HRP Goat Anti-Mouse IgG and HRP Goat Anti-Rabbit IgG, were obtained from ABclonal Technology (Hubei, China). MALAT1-IN-1 and Stattic were purchased from MedChemExpress (NJ, USA). Mibolerone (Mib) was purchased from Sigma Aldrich (MO, USA). Charcoal-stripped serum (CSS) was purchased from Biological Industries (Beit Haemek, Israel). Phenol red-free RPMI 1640 was purchased from Gibco (New York, USA).