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Anti human vegf

Manufactured by R&D Systems
Sourced in United States

Anti-human VEGF is a laboratory reagent used for research purposes. It is a protein that binds to and neutralizes the activity of human vascular endothelial growth factor (VEGF), a key regulator of angiogenesis. This product is intended for use in in vitro experiments and assays to study the role of VEGF in various biological processes.

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2 protocols using anti human vegf

1

Blocking Antibodies and Mcl-1 Inhibitor Treatment

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The following blocking antibodies were used: 10–20 μg/mL anti-mouse GM-CSF blocking antibody (eBioscience, San Diego, CA, USA), 20 μg/mL anti-mouse VEGF, 20 μg/mL anti-mouse IL-10, 10 μg/mL anti-human GM-CSF, 10 μg/mL anti-human VEGF and 20 μg/mL anti-human IL-10 blocking antibodies (R&D systems, Minneapolis, MN, USA). Isotype controls used were 10 μg/mL rat IgG2aK isotype control (eBioscience) and normal goat IgG control (R&D). Mcl-1 inhibitor MIM1 (R&D) was dissolved in DMSO.
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2

Inhibitors of Angiogenesis Signaling Pathways

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Recombinant human VEGF165, RANKL, and macrophage colony-stimulating factor (M-CSF) were purchased from R&D Systems (Minneapolis, MN). Inhibitors of signal molecules such as Src inhibitor (PP1), protein kinase C (PKC) inhibitor (PKC inhibitor peptide) and P38 mitogen-activated protein kinase (MAPK) inhibitor (SB203580) were obtained from Calbiochem (Schwalbach, Germany). Anti-human VEGF, Anti-human VEGFR1, Anti-human VEGFR2 and anti-CD55 antibodies were purchased from R&D Systems.
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