Cep164

Manufactured by Proteintech

CEP164 is a protein that is involved in the function of cilia, which are hair-like structures found on the surface of many cells. It plays a crucial role in the formation and maintenance of cilia. The CEP164 protein is commonly used in research to study cilia-related processes and functions.

Automatically generated - may contain errors

Lab products found in correlation

Centrin, by Merck Group (2 mentions) Cep164, by Santa Cruz Biotechnology (2 mentions) Acetylated α tubulin, by Santa Cruz Biotechnology (2 mentions) Rootletin, by Santa Cruz Biotechnology (1 mentions) Centrin 3, by Abnova (1 mentions) Cep68, by Proteintech (1 mentions) H 1000 10, by Vector Laboratories (1 mentions) γ tubulin, by Merck Group (1 mentions) Ab150116, by Abcam (1 mentions)

Close spelling variants of this product

Rabbit anti-CEP164 (2 citations)

3 protocols using cep164

Immunofluorescence Staining of Centrosomal Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following commercial antibodies were used: CEP290 (C-terminus), Bethyl Laboratory, A301-659A; acetylated α-tubulin, Santa Cruz, sc-23950; Centrin, pan-Centrin antibody from EMD Millipore, 04-1624, raised against Chlamydomonas Centrin and reported to recognize all mouse Centrin isoforms 1-4 (41, 84); CEP164, Proteintech, 22227-1-AP; CEP164, Santa Cruz, sc-515403; MKS3 (cultured cells) Proteintech, 13975-1-AP; Atto488-Tuba1, Antibodies-online.com, ABIN1169085. Antibodies were used at a concentration of 10 μg/mL, with the exception of Atto488-Tuba1 which was used at a concentration of 7.5 μg/mL. Secondary antibodies used were F(ab’)2-goat anti-rabbit IgG Alexa 488, F(ab’)2-goat anti-mouse IgG Alexa 555, and F(ab’)2-goat anti-mouse IgG Alexa 647 (Thermo Fisher, 8 μg each).

Boulton I.C., Yost M.K., Anderson J.E, & Cornelissen C.N. (2000). Identification of Discrete Domains within Gonococcal Transferrin-Binding Protein A That Are Necessary for Ligand Binding and Iron Uptake Functions. Infection and Immunity, 68(12), 6988-6996.

+ Open protocol

+ Expand

Immunofluorescence Staining of Centrosomal Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were grown on coverslips and fixed with ice-cold methanol at -20°C for 10 min. The coverslips were then blocked with %5 BSA (Sigma; A3733) and primary antibodies were diluted in %1 BSA and incubated overnight at 4°C. The secondary antibody incubations were carried out at room-temperature for 1 hour, and washes were performed with PBS. Then coverslips were dried and then mounted on glass slides using a mounting medium containing DAPI (Vector Laboratories; H-1000-10).
The primary antibodies and their concentrations used in the experiments were: Centrin-3 (Abnova; H00001070-M01) at1/500 dilution;

γ

-tubulin (Sigma; T6557) at 1/500 dilution;

γ

-tubulin (Sigma; T3559): 1/250; CEP120 (Atlas Antibodies; HPA028823): 1/500; CEP152 (Bethyl Labs; A302-479A) at 1/500 dilution; CEP164 (Proteintech; 22227-1-AP) at 1/500 dilution; CEP170 (Bethyl Labs; A301-024A) at 1/250 dilution, C-Nap1 (Millipore; MABT1353) at 1/500 dilution; CEP68 (Proteintech; 15147-1-AP) at 1/500 dilution; Rootletin (Santa Cruz Biotechnology; sc-374056) at 1/500 dilution; Nek2 (BD; 610593) at 1/500 dilution. The secondary antibodies used in the experiments were: anti-rabbit AF488 (Invitrogen; A-11008), anti-rabbit AF555 (Invitrogen; A-31572), anti-mouse AF594 (Abcam; ab150116) and anti-mouse AF647 (Abcam; ab150115).

Ozcan S.C., Kalkan B.M., Cicek E., Canbaz A.A, & Acilan C. (2024). Prolonged overexpression of PLK4 leads to formation of centriole rosette clusters that are connected via canonical centrosome linker proteins. Scientific Reports, 14, 4370.

+ Open protocol

+ Expand

Immunofluorescence Staining Protocols

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following commercial antibodies were used CEP290, Bethyl Laboratory, A301-659A; acetylated a-tubulin, Santa Cruz, sc-23950; NPHP5, Proteintech, 15747-1-AP; Centrin, EMD Millipore, 04-1624; CEP164, Proteintech, 22227-1-AP; CEP164, Santa Cruz, sc-515403; wheat germ agglutinin (WGA)-A647, Molecular Probes, W32466; Atto488-Tuba1, Antibodies-online.com, ABIN1169085. Rat anti-CEP290 antibody was a gift from the Swaroop laboratory. Mouse anti-1D4 was generated in house. For SIM and confocal imaging, antibodies were used at a concentration of 1 μg/150 μL. For STORM, antibodies were used at a concentration of 10 μg/mL, with the exception of rat anti-CEP290 antibody and Atto488-Tuba1 which were used at concentrations of 5 μg/mL and 7.5 μg/mL, respectively.

Potter V.L., Moye A.R., Robichaux M.A., & Wensel T.G. (2020). Superresolution microscopy reveals photoreceptor-specific subciliary location and function of Cep290.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!