The following antibodies were used in this study: anti-PD-L1 (Bio X Cell, Lebanon, NH, Cat. No: BE0101, clone: 10F.9G2) and isotype control (Bio X Cell, catalog number: BE0090, clone: LTF-2), anti-IFN-g (Thermo Fisher Scientific, Waltham, MA, catalog number: PHC4031 and ProSpec, Rehovot, Israel, catalog number: CYT-358), rabbit anti-ARID1A (Cell Signaling Technology, Danvers, MA, catalog number: 12354), rabbit anti-ARID1B (Cell Signaling Technology, catalog number: 92964), mouse anti-ARID1B (Santa Cruz Biotechnology, Dallas, TX, catalog number: sc-32762), mouse anti-ARID2 (Santa Cruz Biotechnology, catalog number: sc-166117; Abcam, Cambridge, United Kingdom, catalo number: ab51019), rabbit anti-BRG1 (Cell Signaling Technology, catalog number: 49360), rabbit anti-BAF155 (Cell Signaling Technology, catalog number: 11956), rabbit anti-phosphorylated STAT1 (Cell Signaling, catalog number: 9167), rabbit anti-STAT1 (Cell Signaling Technology, catalog number: 9172), mouse anti-FLAG (Sigma-Aldrich catalog number: F1804), rabbit anti-PD-L1 (Cell Signaling Technology, catalog number: 13684S, Abcam, catalog number: ab213480), and mouse anti-b-actin (Sigma-Aldrich, catalog number: A5441).
Rabbit anti phosphorylated stat1
Manufactured by Cell Signaling Technology
Sourced in United Kingdom
Rabbit anti-phosphorylated STAT1 is a primary antibody that recognizes the phosphorylated form of Signal Transducer and Activator of Transcription 1 (STAT1) protein. STAT1 is a transcription factor that plays a critical role in cellular signaling pathways.
Automatically generated - may contain errors
Lab products found in correlation
Rabbit anti stat1, by Cell Signaling Technology (4 mentions)
Goat anti rabbit igg hrp conjugated secondary antibody, by Cell Signaling Technology (3 mentions)
Pvdf membrane, by Merck Group (3 mentions)
Rabbit anti tyk2, by Abcam (3 mentions)
Hrp conjugated goat anti mouse igg secondary antibody, by ZSGB-BIO (3 mentions)
Mouse anti stat3, by BD (3 mentions)
Rabbit anti phosphorylated stat3, by Cell Signaling Technology (3 mentions)
Anti ifng, by Thermo Fisher Scientific (1 mentions)
Mouse anti β actin, by Merck Group (1 mentions)
Mouse anti flag, by Merck Group (1 mentions)
Anti pd l1, by BioXCell (1 mentions)
Rabbit anti pd l1, by Cell Signaling Technology (1 mentions)
Rabbit anti brg1, by Cell Signaling Technology (1 mentions)
Rabbit anti arid1a, by Cell Signaling Technology (1 mentions)
4 protocols using rabbit anti phosphorylated stat1
1
Antibody Staining Protocol for PD-L1 and ARID Proteins
2
PBMC Cytokine Stimulation Western Blot
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PBMCs were stimulated with various cytokines for indicated time. The extracted protein were subjected to 10% SDS-PAGE and transferred onto PVDF membranes (Millipore). The following primary antibodies were used: rabbit anti-phosphorylated STAT1 (Cell Signaling Technology) and rabbit anti-STAT1 (Cell Signaling Technology), rabbit anti-phosphorylated STAT3 (Cell Signaling Technology), mouse anti-STAT3 (BD), rabbit anti-TYK2(Abcam, cat223733), and that against β-actin rabbit mAb horseradish peroxidase (HRP)-conjugated (Cell Signaling Technology). The HRP-conjugated goat anti-rabbit IgG secondary antibody (Cell Signaling Technology) and the HRP-conjugated goat anti-mouse IgG secondary antibody (ZSGB-BIO, China).
3
Western Blot Analysis of STAT Signaling
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PBMCs were stimulated with various cytokines for indicated time. The extracted protein was subjected to 10% SDS-PAGE and transferred onto PVDF membranes (Millipore). The following primary antibodies were used: rabbit anti-phosphorylated STAT1 (Cell Signaling Technology) and rabbit anti-STAT1 (Cell Signaling Technology), rabbit anti-phosphorylated STAT3 (Cell Signaling Technology), mouse anti-STAT3 (BD), rabbit anti-TYK2(Abcam, cat223733), and that against β-actin rabbit mAb horseradish peroxidase (HRP)conjugated (Cell Signaling Technology). The HRP-conjugated goat anti-rabbit IgG secondary antibody (Cell Signaling Technology) and the HRP-conjugated goat anti-mouse IgG secondary antibody (ZSGB-BIO, China).
4
PBMC Cytokine Stimulation Western Blot
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
PBMCs were stimulated with various cytokines for indicated time. The extracted protein were subjected to 10% SDS-PAGE and transferred onto PVDF membranes (Millipore). The following primary antibodies were used: rabbit anti-phosphorylated STAT1 (Cell Signaling Technology) and rabbit anti-STAT1 (Cell Signaling Technology), rabbit anti-phosphorylated STAT3 (Cell Signaling Technology), mouse anti-STAT3 (BD), rabbit anti-TYK2(Abcam, cat223733), and that against β-actin rabbit mAb horseradish peroxidase (HRP)-conjugated (Cell Signaling Technology). The HRP-conjugated goat anti-rabbit IgG secondary antibody (Cell Signaling Technology) and the HRP-conjugated goat anti-mouse IgG secondary antibody (ZSGB-BIO, China).
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