FTase and GGTase I activity was determined using the method of Temple et al.,48 (link) with modifications as previously described.22 (link) Recombinant FTase and GGTase I were obtained from Jena Biosciences and dansyl-GCVLS and dansyl-GCVLL were obtained from Biosynthesis, Inc. Changes in fluorescence over time were detected using a Molecular Devices Spectramax Gemini EM fluorescence microplate reader. Compounds were tested in duplicate at multiple concentrations and three independent experiments were performed.
Dansyl gcvll
Manufactured by Bio-Synthesis
Sourced in United States
Dansyl-GCVLL is a chemical compound that can be used as a fluorescent labeling reagent. It is composed of the dansyl group and the amino acid sequence GCVLL. The core function of Dansyl-GCVLL is to provide a fluorescent label that can be used to detect and track the presence of specific proteins or peptides in various analytical and experimental applications.
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Lab products found in correlation
Spectramax gemini em, by Molecular Devices (1 mentions)
Dansyl gcvls, by Bio-Synthesis (1 mentions)
Anti β actin, by Merck Group (1 mentions)
Triton x 100, by Merck Group (1 mentions)
Tween 20, by Merck Group (1 mentions)
Ab5622, by Merck Group (1 mentions)
Mab5258, by Merck Group (1 mentions)
Anti synaptotagmin, by Abcam (1 mentions)
Filipin 3, by Merck Group (1 mentions)
Mab1598, by Merck Group (1 mentions)
Phosphatase inhibitor cocktail 04906837001, by Roche (1 mentions)
Ggti 2133, by Merck Group (1 mentions)
Anti p trk, by Santa Cruz Biotechnology (1 mentions)
24 s hydroxycholesterol, by Merck Group (1 mentions)
Sc 30193, by Santa Cruz Biotechnology (1 mentions)
2 protocols using dansyl gcvll
1
Enzymatic Activity Assay for FTase and GGTase I
2
Immunohistochemical Characterization of Neuronal Markers
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The following primary antibodies were used: anti-FLAG®M2 (clone M2, F1804; Sigma-Aldrich Inc., St Louis, MO, USA), anti-microtubule-associated protein 2 (MAP2) (AB5622, Merck Millipore Corporation., Darmstadt, Germany), anti-postsynaptic density protein 95 (PSD95) (MAB1598, Merck Millipore), anti-SH3 and multiple ankyrin repeat domains 3 (Shank3) (sc-30193, Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA), anti-Synaptotagmin (ab77314, Abcam, Cambridge, UK) anti-Synaptophysin (MAB5258, Merck Millipore), anti p-Trk (sc-8058; Santa Cruz), anti-GGTase-Iβ (sc-1899, Santa Cruz), anti-TrkB (sc-12, Santa Cruz) and anti-β-actin (A5541, Sigma). Geranylgeranyl pyrophosphate (GGPP) ammonium salt, Triton™ X-100, Tween® 20, IGEPAL®, dimethyl sulfoxide (DMSO), filipin III, 24S-hydroxycholesterol and the chemical inhibitors GGTi-2133 and K252a were all purchased from Sigma. The phosphatase inhibitor cocktail (04906837001) and the protease inhibitor cocktails 1 and 2 (11697498001 and 11836170001, respectively) were purchased from Roche Diagnostics (GmbH, Penzberg, Germany). The fluorogenic substrate used for GGTase-I activity assay was dansyl-GCVLL (Biosynthesis, Lewisville, TX, USA). Phalloidin was obtained from Molecular Probes®, Thermo Fisher Scientific Inc. (Waltham, MA, USA).
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