Bromazepam

Sample preparation was performed with analytical grade solvents obtained from Merck KGaA (Darmstadt, Germany). LC-MS grade acetonitrile for the mobile phase was obtained from Fisher Scientific GmbH (Schwerte, Germany), and water and formic acid (99% purity) were purchased from Acros Organics (Geel, Belgium). The following deuterated standards (99% purity) were purchased from LGC Standards GmbH (Wesel, Germany): 6 Furthermore, the following standards (99% purity) were purchased from LGC Standards GmbH (Wesel, Germany) or Sigma Aldrich (St. Louis, MO): 7-aminoflunitrazepam, alprazolam, amisulpride, amitriptyline, aripiprazole, benperidol, bromazepam, bupropion, chlorpromazine, chlorprothixene, citalopram, clomipramine, clonazepam, clozapine, diazepam, doxepin, flunitrazepam, fluoxetine, fluphenazine, flupentixol, f l u v o x a m i n e , h a l o p e r i d o l , h y d r o x y r i s p e r i d o n e , levomepromazine, maprotiline, melperone, methylphenidate, mianserin, midazolam, mirtazapine, norcitalopram, norclomipramine, norclozapine, nordiazepam, nordoxepin, nortriptyline, norvenlafaxine, olanzapine, opipramol, oxazepam, paroxetine, pipamperone, promethazine, quetiapine, sulpiride, temazepam, trazodone, trimipramine, venlafaxine, and zuclopenthixol. A standard mixture containing 10 ng/μL of each analyte in methanol was prepared and diluted as required.