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Nonafluoropentanoic acid

Manufactured by Merck Group
Sourced in United States

Nonafluoropentanoic acid is a synthetic organic compound with the chemical formula C5HF9O2. It is a colorless, crystalline solid with a high degree of fluorination, which gives it unique physical and chemical properties. The core function of this compound is to serve as a specialty chemical and laboratory reagent, finding applications in various fields of research and analysis.

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2 protocols using nonafluoropentanoic acid

1

HPLC Protocol for Amino Acid Analysis

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A Millipore–Direct Q3 UV System (Molsheim, France) was used to obtain Milli-Q® water (18.2 MΩ·cm). For the HPLC analysis, the reagents Chloride acid (HCl), formic acid (98% p.a), and acetonitrile HPLC gradient grade were obtained from Carlo Erba Reagents SAS (Val de Reuil, France). Amino acids standard H was purchased from Thermo Fisher Scientific (Rockford, MA, USA), and the phenol BioXtra ≥ 99.5% (GC), nonafluoropentanoic acid, bovine trypsin from bovine pancreas ≥ 10,000 BAEE units/mg protein, calcium chloride dehydrated (CaCl2·2H2O), benzoyl-L-arginine-p-nitroanilide (L-BAPA), dimethyl sulfoxide (DMSO), tris(hydroxymethyl)aminomethane, glacial acetic acid (≥99%), sodium hydroxide (NaOH), porcine trypsin type IX-S, bovine α-chymotrypsin, type II, Streptomyces griseus protease, type XIV and casein from bovine milk were purchased from Sigma-Aldrich (St. Louis, MI, USA).
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2

Antioxidant and Glycation Assay Reagents

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2,2-Diphenyl-1-picrylhydrazyl (DPPH • ), 2,2-azinobis-3-ethyl benzothiazoline-6-sulphonic acid (ABTS), 6-hydroxy-2,5,7,8,-tetramethylchromane-2-carboxylic acid (Trolox), 2,2'-azobis(2methylpropionamidine) dihydrochloride (AAPH), diethylene glycol, sodium borohydride, boric acid, nonafluoropentanoic acid (NFPA), trichloroacetic acid (TCA), D (+)-lactose monohydrate, acetonitrile (HPLC grade), rutin, quercetin-3-O-glucoside, quinic acid, p-coumaric, caffeic and gallic acids, Folin-Ciocalteu phenol reagent and Milli-Q water were from Sigma-Aldrich (St.
Louis, MO, USA). (+)-Catechin reference was purchased from Chromadex (Irvine, California, USA), chlorogenic acid, L-phenylalanine and glucose were from Roth (Karlsruhe, Germany); fluorescein sodium salt (FL) from Fluka Chemicals (Steinheim, Germany). Heterocyclic amines,
and PhIP were purchased from Toronto Research Chemicals Inc. (Toronto, Canada). CML, d2-CML and furosine were purchased from PolyPeptide Laboratories (Strasbourg, France).
Creatinine was from Alfa Aesar (Karlsruhe, Germany). Bond Elut PRS LRC cartridges and Bond-Elut C18 cartridges were purchased from Agilent Technologies (Lake Forest, CA, USA).
Chromabond XTR cartridges were from Macherey-Nagel GmbH & Co. (Bethlehem, PA, USA).
Oasis HLB cartridges for CML and furosine stable isotope dilution assay were purchased from Waters Corporation (Milford, CA, USA).
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