Intercepted bone mass was thawed, fixed in 10% neutral formalin for 48 hours, and embedded in paraffin after decalcification in 10% EDTA solution (Zhongshan Jinqiao, China). The specimens were then cut into 5 μm thick sections and treated with 3% hydrogen peroxide for 10 min. Afterward, the sections were rinsed with phosphate-buffered saline, incubated with primary and secondary antibodies (Abcam, UK., the same as WB) sequentially, and exposed to DAB (TaKaRa, Japen). The sections were counterstained with hematoxylin solution (TaKaRa, Japen). Ten visual fields were randomly selected and observed under a high magnification microscope (Olympus, Japen), and the number of positively stained cells was calculated.
Hematoxylin solution
Manufactured by Takara Bio
Sourced in China
Hematoxylin solution is a staining reagent commonly used in histology and cytology laboratories. Its core function is to stain cell nuclei blue, allowing for the visualization and identification of cellular structures under a microscope.
Automatically generated - may contain errors
2 protocols using hematoxylin solution
1
Immunohistochemical Analysis of Bone Cells
2
Bone Histology Analysis Protocol
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Intercepted bone mass was thawed, xed in 10% neutral formalin for 48 hours, and embedded in para n after decalci cation in 10% EDTA solution (Zhongshan Jinqiao, Beijing, China). The tissues were then cut into 5-µm thick sections and treated with 3% hydrogen peroxide for 10 minutes. Afterward, the sections were rinsed with phosphate-buffered saline, incubated with primary and secondary antibodies (Bio-Rad, Hercules, USA) sequentially, and exposed to DAB (TaKaRa, Dalian, China). The sections were counterstained with hematoxylin solution (TaKaRa, Dalian, China). Ten visual elds were randomly selected and observed under a high magni cation microscope (400×) (Leica DM500, Solms,germany), and the number of positively stained cells was calculated.
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