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Zymo rna clean and concentrator 5 columns

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The Zymo RNA Clean and Concentrator-5 columns are designed to purify and concentrate RNA samples. The product features rapid spin-column technology to efficiently remove contaminants and concentrate RNA samples for downstream applications.

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Lab products found in correlation

Agilent tapestation, by Agilent Technologies (2 mentions) Dnase 1, by New England Biolabs (2 mentions)

Close spelling variants of this product

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2 protocols using zymo rna clean and concentrator 5 columns

1

RNA-Seq Analysis of SH-SY5Y Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
For preliminary RNA-Seq of SH-SY5Y cells, eight replicate sub-confluent (approximately 80%) 10 cm dishes (TPP) were harvested by dissociation from the plate by pipetting and centrifugation at 500 × g, 5 min at room temperature. Pellets were lysed according to TRAP protocol lysis conditions (Methods S2), and RNA was purified using Trizol LS, followed by treatment for 15 min at 37°C with DNase I (NEB) and cleanup using Zymo RNA Clean and Concentrator-5 columns (Zymo Research). RNA samples were assessed by Agilent TapeStation with RINe values between 8–10. RNA was fragmented and prepared into libraries for next generation sequencing using Methods S1 as for CLIP-Seq samples.
Rieger M.A., King D.M., Crosby H., Liu Y., Cohen B.A, & Dougherty J.D. (2020). CLIP and Massively Parallel Functional Analysis of CELF6 Reveal a Role in Destabilizing Synaptic Gene mRNAs through Interaction with 3′ UTR Elements. Cell reports, 33(12), 108531.
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2

RNA-Seq Analysis of SH-SY5Y Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
For preliminary RNA-Seq of SH-SY5Y cells, eight replicate sub-confluent (approximately 80%) 10 cm dishes (TPP) were harvested by dissociation from the plate by pipetting and centrifugation at 500 × g, 5 min at room temperature. Pellets were lysed according to TRAP protocol lysis conditions (Methods S2), and RNA was purified using Trizol LS, followed by treatment for 15 min at 37°C with DNase I (NEB) and cleanup using Zymo RNA Clean and Concentrator-5 columns (Zymo Research). RNA samples were assessed by Agilent TapeStation with RINe values between 8–10. RNA was fragmented and prepared into libraries for next generation sequencing using Methods S1 as for CLIP-Seq samples.
Rieger M.A., King D.M., Crosby H., Liu Y., Cohen B.A, & Dougherty J.D. (2020). CLIP and Massively Parallel Functional Analysis of CELF6 Reveal a Role in Destabilizing Synaptic Gene mRNAs through Interaction with 3′ UTR Elements. Cell reports, 33(12), 108531.
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