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Minimal microbial growth medium m9 media

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Minimal microbial growth medium (M9 media) is a laboratory culture medium used to support the growth of microorganisms. It provides the essential nutrients and minerals required for basic cellular functions and growth.

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Lab products found in correlation

Protein g magnetic beads, by Thermo Fisher Scientific (2 mentions) Ni nta agarose resin, by Qiagen (2 mentions) 13c615n2 lysine, by Cambridge Isotopes (2 mentions) 13c615n1 leucine, by Cambridge Isotopes (2 mentions) Dl dithiothreitol, by Merck Group (2 mentions) Imidazole, by Merck Group (2 mentions) Ethanolamine, by Merck Group (2 mentions) Human lysozyme, by Merck Group (2 mentions) Triethanolamine, by Merck Group (2 mentions) Minimal salts, by Merck Group (2 mentions) Bovine serum albumin (bsa), by Merck Group (2 mentions) Ammonium bicarbonate, by Thermo Fisher Scientific (1 mentions) Acetic acid, by Thermo Fisher Scientific (1 mentions) Glycerol, by Merck Group (1 mentions) Dimethyl pimelimidate dihydrochloride dmp, by Merck Group (1 mentions) Ethylenediaminetetraacetic acid, by Merck Group (1 mentions) Phenylmethylsulfonyl fluoride pmsf, by Merck Group (1 mentions) Edta free protease inhibitor cocktail, by Merck Group (1 mentions)

2 protocols using minimal microbial growth medium m9 media

1

Quantitative Proteomic Analysis of Frataxin

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All reagents and solvents were LC-MS grade quality unless otherwise noted. [13C615N2]-lysine and [13C615N1]-leucine were from Cambridge Isotope Laboratories (Andover, MA, United States). Anti-frataxin mouse monoclonal antibody [17A11] Ab113691 was from Abcam (Cambridge, MA, United States). Dimethyl pimelimidate dihydrochloride (DMP), Ethylenediaminetetraacetic acid (EDTA), cOmplete™ Mini EDTA-free Easypack protease inhibitor cocktail tablets, endoproteinase Asp-N sequencing grade, DL-dithiothreitol (DTT), bovine serum albumin (BSA), human lysozyme, imidazole, glycerol, phenylmethylsulfonyl fluoride (PMSF), triethanolamine, ethanolamine, and M9, minimal salts, 5X powder, minimal microbial growth medium (M9 media) were purchased from MilliporeSigma (Billerica, MA, United States). Ni-NTA agarose resin was purchased from Qiagen (Germantown, MD, United States). LC grade water and acetonitrile were from Burdick and Jackson (Muskegon, MI, United States). Ammonium bicarbonate and acetic acid were purchased from Fisher Scientific (Pittsburgh, PA, United States). Protein G magnetic beads were obtained from Life Technologies Corporation (Grand Island, NY, United States).
Wang Q., Laboureur L., Weng L., Eskenazi N.M., Hauser L.A., Mesaros C., Lynch D.R, & Blair I.A. (2022). Simultaneous Quantification of Mitochondrial Mature Frataxin and Extra-Mitochondrial Frataxin Isoform E in Friedreich’s Ataxia Blood. Frontiers in Neuroscience, 16, 874768.
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2

Affinity Purification of Frataxin Protein

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Reagents and solvents were LC-MS grade quality unless otherwise noted. [13C615N2]-lysine and [13C615N1]-leucine were from Cambridge Isotope Laboratories (Andover, MA, USA). Antifrataxin antibody (clone 1D9) was from LifeSpan Biosciences, Inc. (Seattle, WA). Dimethyl pimelimidate dihydrochloride (DMP), EDTA-free protease inhibitor cocktail, d,l-dithiothreitol (DTT), isopropyl-β-d-thiogalactoside (IPTG), bovine serum albumin (BSA), human lysozyme, imidazole, triethanolamine, ethanolamine, and M9, minimal salts, 5× powder, minimal microbial growth medium (M9 media) were purchased from MilliporeSigma (Billerica, MA). Ni-NTA Agarose resin was purchased from Qiagen (Germantown, MD). LC grade water and acetonitrile were from Burdick and Jackson (Muskegon, MI, USA). Protein G magnetic beads were obtained from Life Technologies Corporation (Grand Island, NY).
Guo L., Wang Q., Weng L., Hauser L.A., Strawser C.J., Rocha A.G., Dancis A., Mesaros C., Lynch D.R, & Blair I.A. (2018). Liquid Chromatography-High Resolution Mass Spectrometry Analysis of Platelet Frataxin as a Protein Biomarker for the Rare Disease Friedreich’s Ataxia. Analytical chemistry, 90(3), 2216-2223.
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