Manual spme fiber holder

The aroma of the P. clusii subsp. rhodia red seeds was studied through HS-SPME/GC-MS analyses [64 (link)]. HS-SPME was performed with Supelco SPME fiber 100 μm PDMS (polydimethylsiloxane coating) and Supelco SPME fiber 75 μm PDMS/DVB (polydimethylsiloxane/divinylbenzene coating) attached to a manual SPME fiber holder (Supelco, Bellefonte, PA, USA). First, the fiber was conditioned in the GC at 250 °C for 30 min and then was inserted into the sample vial. For SPME extraction, 500 mg of the sample in a glass vial (15 mL), closed with a PTFE-coated silicone rubber septum, was used. The temperature in our experiment was set at 60 °C and the vial with the sample was placed on the hotplate for 30 min. After that time, the fiber was exposed to the sample for 30 min at 60 °C and then it was transferred to perform GC-MS analysis. The initial temperature of the column was 55 °C for 2 min with an increase of 5 °C/min until it reached 240 °C, where it remained for 2 min. The total analysis time was 41 min. The substances were identified by comparing the mass spectrum of each substance with those of the Wiley 275 library and the literature.

An 85 μm Carboxen/Polydimethylsiloxane (CAR/PDMS) fiber attached to a manual SPME fiber holder (Supelco, Bellefonte, PA) was used for the HS-SPME procedure. Conditioning of the fiber was conducted prior to the analysis based on the instructions of the manufacturer. For the extraction of the volatile compounds, an aliquot of truffle (100 mg) was weighted into a 15 ml-glass vial. Extraction of the volatile compounds took place at 50°C within 45 min. After this time span, the fiber was removed from the vial containing the sample and the analytes were desorbed in the injector of the GC-MS instrument for 5 min which previously had been shown to be sufficient for complete desorption, and for the avoidance of sample carry-over. Each experiment was performed in triplicate.