Enterococcus faecalis mtcc 439

Manufactured by Microbial Type Culture Collection

Enterococcus faecalis MTCC 439 is a microbiological culture maintained by the Microbial Type Culture Collection. It is a strain of the bacterium Enterococcus faecalis.

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2 protocols using enterococcus faecalis mtcc 439

Antibacterial Activity of Probiotic Cultures

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The pure culture of each isolate was grown in MRS broth for 24 h and cell free supernatant (CFS) was obtained by centrifuging at 10,000 rpm, 4 °C, for 10 min. The collected CFS was checked for their antibacterial activity against Enterococcus faecalis MTCC 439, Shigella flexneri MTCC 1457, Aeromonas hydrophila MTCC 1739, Escherichia coli MTCC 40, Pseudomonas aeruginosa MTCC 4679, Staphylococcus aureus MTCC 737, Klebsiella pneumonaie MTCC 39, and Proteus vulgaris MTCC 426 received from Microbial Type Culture Collection (MTCC), Chandigarh, India and clinical pathogens such as P. aeruginosa, K. pneumoniae, Acinetobacter baumannii, methicillin resistant S. aureus (MRSA) received from Mahathma Gandhi Memorial Government Hospital, Tiruchirappalli, Tamil Nadu, India. The assay was performed by well diffusion method using 50 µL of each indicator bacteria (2.1 × 10⁵ CFU/mL).

Duraisamy S., Husain F., Balakrishnan S., Sathyan A., Subramani P., Chidambaram P., Arokiyaraj S., Al-Qahtani W.H., Rajabathar J, & Kumarasamy A. (2022). Phenotypic Assessment of Probiotic and Bacteriocinogenic Efficacy of Indigenous LAB Strains from Human Breast Milk. Current Issues in Molecular Biology, 44(2), 731-749.

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Antimicrobial Potential of Marine Bacteria

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Antimicrobial potential of marine fish bacterial isolates was evaluated against common fish pathogens such as Aeromonas hydrophila, Vibrio alginolyticus, Vibrio parahaemolyticus and Staphylococcus aureus were obtained from Central Marine Fisheries Research Institute (CMFRI), Mandapam, Tamil Nadu, India, and other strains, Shigella flexneri MTCC 1457, Enterococcus. faecalis MTCC 439, A. hydrophila MTCC 1739, Escherichia coli MTCC 40, Pseudomonas aeruginosa MTCC 4679, S. aureus MTCC 737, Klebsiella pneumoniae MTCC 39 and Proteus vulgaris MTCC 426 were purchased from Microbial Type Culture Collection (MTCC), Chandigarh, India.
The Bacillus subtilis culture was purified from the commercially available fish probiotic product and confirmed by morphological and biochemical characterization. A single colony of purified B. subtilis was cultured and used as positive control.

Husain F., Duraisamy S., Balakrishnan S., Ranjith S., Chidambaram P, & Kumarasamy A. (2022). Phenotypic assessment of safety and probiotic potential of native isolates from marine fish Moolgarda seheli towards sustainable aquaculture. Biologia, 77(3), 775-790.

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