All chemicals and solvents were purchased from commercial sources and were used as received, unless otherwise noted. Valproic acid, β-cyclodextrin, MTT (3-[4,5-dimethylthiazol-2-yl]-2,5- diphenyltetrazolium bromide; thiazolyl blue), cetyltrimethyl ammonium bromide (CTAB), tetraethyl orthosilicate (TEOS), (3-aminopropyl)triethoxysilane (APTES), 3-iodopropyltrimethoxysilane (IPTMS), benzimidazole, toluene (anhydrous), fluorescein, 2′,7′-dichlorofluorescein diacetate (DCFDA), Hoechst 33258, calcein blue, and propidium iodide (PI) were obtained from Sigma (St. Louis, MO). Dulbecco's modified Eagle's medium (DMEM), fetal bovine serum (FBS), penicillin, and streptomycin were obtained from Gibco BRL (Grand Island, NY). Antibodies for P53, caspase-3, and PARP were obtained from Cell Signaling Technology (Danvers, MA). Monoclonal antibodies for Bcl-2 (50E3) and PARP (46D11) were purchased from Santa Cruz Biotech (Santa Cruz, CA). Monoclonal antibody for Bax (ab5714) was from Abcam (Cambridge, UK). Bicinchoninic acid (BCA) protein assay kit and ECL chemiluminescence system were obtained from Pierce (Rockford, IL) and GE Healthcare (Piscataway, NJ), respectively. Annexin V–fluorescein isothiocyanate (FITC) Apoptosis Analysis Kit (AO2001-02P-H) was purchased from Sungene Biotech (Tianjin, China).
Annexin 5 fluorescein isothiocyanate fitc apoptosis analysis kit
Manufactured by Sungene Biotech
The Annexin V–fluorescein isothiocyanate (FITC) Apoptosis Analysis Kit is a laboratory reagent used to detect and quantify apoptosis (programmed cell death) in cell populations. It contains Annexin V, a protein that binds to phosphatidylserine, a molecule that is exposed on the surface of cells undergoing apoptosis. The Annexin V is conjugated with the fluorescent dye FITC, allowing for the detection and analysis of apoptotic cells using flow cytometry or fluorescence microscopy.
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Lab products found in correlation
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Propidium iodide, by Merck Group (1 mentions)
Toluene, by Merck Group (1 mentions)
Hoechst 33258, by Merck Group (1 mentions)
Antibodies for p53, by Cell Signaling Technology (1 mentions)
Valproic acid, by Merck Group (1 mentions)
2 7 dichlorofluorescein diacetate, by Merck Group (1 mentions)
Caspase 3, by Cell Signaling Technology (1 mentions)
Benzimidazole, by Merck Group (1 mentions)
β cyclodextrin, by Merck Group (1 mentions)
Calcein blue, by Merck Group (1 mentions)
2 protocols using annexin 5 fluorescein isothiocyanate fitc apoptosis analysis kit
1
Cytotoxicity and Apoptosis Assays for Valproic Acid
2
Annexin V-FITC Apoptosis Assay
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The apoptosis of cumulus cells was detected according to the instructions of the Annexin V‐Fluorescein isothiocyanate (FITC) Apoptosis Analysis Kit (Tianjin Sungene Biotech Co. Ltd.). In brief, cells were treated with trypsin and washed twice with phosphate‐buffered saline (PBS). Then, the cells were centrifuged at 800 × g for 5 min at 4°C and three times. Then, 100 μl of PBS was added to each centrifuge tube, followed by incubation for 15 min with 5 μl of FITC‐labeled Annexin V solution and 5 μl of propidium iodide (20 μg/ml) in the dark. Apoptotic and viable cells were distinguished by staining with propidium iodide and FITC‐labeled annexin V, respectively. The cell samples were then analysed using a flow cytometer (Beckman Coulter).
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