Mouse anti alpha tubulin t6199

Manufactured by Merck Group

Sourced in United States

Mouse anti-alpha-tubulin (T6199) is an antibody that binds to the alpha-tubulin subunit of microtubules. It is used for the detection and quantification of alpha-tubulin in various applications such as Western blotting, immunohistochemistry, and immunocytochemistry.

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2 protocols using mouse anti alpha tubulin t6199

Immunodetection of HSPB8 and autophagy

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Mouse anti-HSPB8 (MAB4987) was from R&D System-Biotechne (Minneapolis, MN, USA). Rabbit anti-HSPB8 (PA5-76780) and rabbit anti-SQSTM1/p62 (PA5-20839) were from ThermoFisher Scientific (Waltham, MA, USA). Rabbit anti-E-cadherin (#3195), rabbit anti-N-cadherin (#13116), rabbit anti-vimentin (#5741), rabbit anti-ERK1/2 (#4695), rabbit anti-pERK1/2 (#4370), rabbit anti-cyclin D (#2926), rabbit anti-CDK4 (#12790), rabbit anti-Akt1 (#2938), rabbit anti-pAkt (Ser473) (#9271), rabbit anti-mTOR (#2983), rabbit anti-p-mTOR (Ser 2448) (#5536), rabbit anti-ATG5 (#12994) were from Cell Signaling (Danvers, MA, USA). Rabbit anti-BRAFV600E (RM8) was from RevMAb Biosciences (San Francisco, CA, USA). Mouse anti-pan-RAS (C-4) (sc-166691) was from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Rabbit anti-LC3 (L8918) and mouse anti-alpha-tubulin (T6199) were from Sigma–Aldrich (St. Louis, MO, USA). Rabbit and mouse Horseradish-peroxidase-conjugated secondary antibody were from Cell Signaling. 3-Methyladenine (3-MA) (S2767) was from Selleckchem (Munich, Germany). Chloroquine (CQ) (C6628) was from Sigma–Aldrich.

Cristofani R., Piccolella M., Montagnani Marelli M., Tedesco B., Poletti A, & Moretti R.M. (2022). HSPB8 counteracts tumor activity of BRAF- and NRAS-mutant melanoma cells by modulation of RAS-prenylation and autophagy. Cell Death & Disease, 13(11), 973.

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Immunodetection of Junctional Proteins

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Catalogue numbers for commercially available antibodies are indicated in parentheses. The rat monoclonal antibody against ZO-1 clone R40.76 was used (21 (link)). Antibodies against Claudin-1 (71–7800), Claudin-3 (34–1700), Rab11a (71–5300) and Rhodamine phalloidin were from Thermo Fisher Scientific (Waltham, MA). The rabbit polyclonal antibody against Par-3 was from Milipore (07–330; Billerica, MA). The rabbit polyclonal antibody against Bsep was from Kamiya Biomedical Company (MC-333; Tukwila, WA). The rabbit antibody against cingulin was a gift from Dr. Sandra Citi (University of Geneva) (22 (link)) and the Myosin Vb was from Dr. John Hammer (NHLBI, NIH) (23 (link)). Rabbit anti-LKB1 (CST3047), anti-AMPK (CST2532), anti-phospho-Thr172 AMPK (pAMPK; CST2535) antibodies were purchased from Cell Signaling Technology (Danvers, MA). Mouse anti-alpha-tubulin (T6199) was from Sigma-Aldrich.

Porat‐Shliom N., Tietgens A.J., Van Itallie C.M., Vitale‐Cross L., Jarnik M., Harding O.J., Anderson J.M., Gutkind J.S., Weigert R, & Arias I.M. (2016). Liver kinase B1 regulates hepatocellular tight junction distribution and function in vivo. Hepatology (Baltimore, Md.), 64(4), 1317-1329.

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