Proteins from cells were lysed with RIPA Lysis and Extraction Buffer (Thermos Fisher Scientific, Waltham, MA, USA). After being mixed with Laemmli buffer (BioRad, Hercules, CA, USA), protein samples were heated at 95 °C for 10 min. Protein samples were then separated by SDS-PAGE gel electrophoresis, and transferred onto PVDF membrane (Millipore, Billerica, MA, USA). After being probed with primary and secondary antibodies, protein bands in membranes were detected for chemiluminescence using SuperSignal™ West Pico Chemiluminescent Substrate (Thermo Fisher, Rockford, lL, USA). The primary antibodies used were: anti-p21Cip1 (#2947) and anti-phospho-SAMHD1 (Thr592) (#89930) (Cell Signaling Technologies, Danvers, MA, USA); anti-SAMHD1 (#12586–1-AP), anti-GAPDH (#60004–1-Ig) and anti-RRM2 (#11661–1-AP) (Proteintech Group, Inc. Rosemont, IL, USA); anti-p53 (#sc-126, Santa Crus, Dallas, TX, USA); anti-actin (#A5441, Sigma-Aldrich, St. Louis, MO, USA) and anti-tubulin (# N-356, Amersham, GE Healthcare, Pittsburgh, PA, USA). Western blot images were detected by the ChemiDoc XRS+ system (BioRad, Hercules, CA, USA), and image analysis was performed by using the Image Lab™ software (BioRad, Hercules, CA, USA).
Anti rrm2
Manufactured by Proteintech
Sourced in United States, China
Anti-RRM2 is a laboratory reagent used for the detection and analysis of the RRM2 (Ribonucleotide Reductase Regulatory Subunit M2) protein. RRM2 is a key component of the ribonucleotide reductase enzyme complex, which plays a crucial role in DNA synthesis and repair. Anti-RRM2 can be used in various experimental techniques, such as Western blotting, immunohistochemistry, and immunoprecipitation, to study the expression and localization of the RRM2 protein.
Automatically generated - may contain errors
Lab products found in correlation
Anti p21cip1, by Cell Signaling Technology (1 mentions)
Laemmli buffer, by Bio-Rad (1 mentions)
Anti actin, by Merck Group (1 mentions)
Anti phospho samhd1 thr592, by Cell Signaling Technology (1 mentions)
Anti samhd1, by Proteintech (1 mentions)
Anti tubulin, by Cytiva (1 mentions)
Ripa lysis and extraction buffer, by Thermo Fisher Scientific (1 mentions)
Pvdf membrane, by Merck Group (1 mentions)
Supersignal west pico chemiluminescent substrate, by Thermo Fisher Scientific (1 mentions)
Image lab software, by Bio-Rad (1 mentions)
Anti gapdh, by Proteintech (1 mentions)
Chemidoc xrs system, by Bio-Rad (1 mentions)
Polyvinylidene fluoride membrane, by Merck Group (1 mentions)
Horseradish peroxidase hrp conjugated goat anti rabbit igg, by Proteintech (1 mentions)
Hrp conjugated goat anti mouse igg, by Proteintech (1 mentions)
Anti cdk2, by Proteintech (1 mentions)
Anti myc, by Cell Signaling Technology (1 mentions)
Anti ephb4, by Cell Signaling Technology (1 mentions)
Anti eya3, by Proteintech (1 mentions)
Phenyl methyl sulfonyl fluoride (pmsf), by Merck Group (1 mentions)
2 protocols using anti rrm2
1
Western Blot Analysis of Cell Signaling Proteins
2
Western Blot Analysis of HUVEC Proteins
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Total proteins in HUVECs were extracted with radioimmunoprecipitation assay (RIPA) b00uffer (Beyotime, Shanghai, China) containing phenylmethylsulfonyl fluoride (PMSF) (1 mM) (Sigma-Aldrich) and quantified using a bicinchoninic acid (BCA) kit (Beyotime). Protein extracts were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and electroblotted onto polyvinylidene fluoride membranes (Millipore, Billerica, MA, USA). The resulting membranes were probed with primary antibodies, followed by the corresponding secondary antibodies. The primary antibodies used were anti-EFNB2 (1:1,000) (SAB, Greenbelt, Maryland), anti-GJA4 (1:1,000) (SAB), anti-EPHB4 (1:800) (Cell Signaling, Boston, MA), anti-MYC (1:1,000) (Cell Signaling), anti-EYA3 (1:1,000) (Proteintech, Wuhan, China), anti-CDK2 (1:1,000) (Proteintech), anti-RRM2 (1:1,000) (Proteintech), anti-MYC (pT58) (1:1,000) (SAB), anti-MYC (pS62) (1:1,000) (SAB), and anti-β-ACTIN (1:5,000) (Proteintech). The secondary antibodies included horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG and HRP-conjugated goat anti-mouse IgG (1:5,000) (Proteintech). The bands were detected using a chemiluminescence system (Tanon, Shanghai, China). Quantification was performed using ImageJ2 (Rawak Software, Stuttgart, Germany).
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