Anti rabbit igg whole molecule

Rabbit and mouse primary antibodies against human, namely GRP78 and IRE1α, were provided by the Cell Signaling Technology, Co. (Beverly, MA, USA). Resveratrol (Cas Number: 501-36-0) was purchased from Sigma-Aldrich Co. (Oakville, ON, Canada). GAPDH was prepared from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA), and anti-rabbit IgG (whole molecule) and anti-mouse immunoglobulin G (IgG) (Fab specific) as secondary antibodies were provided by Sigma-Aldrich (Oakville, ON, Canada). The bicinchoninic acid (BCA) protein assay kit was provided by Thermo Fisher Scientific (Winnipeg, MB, Canada).
The enhanced chemiluminescence (ECL) western blot (high sensitivity) substrate kit (ab133406) was obtained from Abcam (Cambridge, MA, USA).

5-FU (50 mg/mL) and RPMI 1640 culture medium were bought from Ebewe Pharma (Belgium) and Invitrogen (Carlsbad, CA, USA), respectively. The D4476, CK1α inhibitor D4476 (ab120220) was obtained from Abcam (Cambridge, MA, USA), and its 10 mM stock solution was kept in DMSO at − 20 °C. Antibodies against human p62 (88588S), LC3B (L7543), and Glycerinaldehyde-3-phosphate-dehydrogenase (GAPDH) (sc-47724) were bought from the cell Signaling Technology (Beverly, MA, USA); Sigma-Aldrich (St. Louis, MO, USA) and Santa Cruz Biotechnology (California, USA), respectively. Anti-rabbit IgG (whole molecule) and anti-mouse IgG (Fab specific) peroxidase conjugated secondary antibodies were provided from Sigma-Aldrich (St. Louis, MO, USA).