Apoe2

Manufactured by Thermo Fisher Scientific

The ApoE2 is a lab equipment product designed for the analysis and quantification of apolipoprotein E (ApoE) in biological samples. It serves as a tool for researchers and scientists working in the fields of lipid metabolism, cardiovascular disease, and neurodegenerative disorders.

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Lab products found in correlation

X vivo 15 media, by Lonza (1 mentions) Maxisorp, by Thermo Fisher Scientific (1 mentions) Apoa 1, by Merck Group (1 mentions) Apoc3, by Merck Group (1 mentions) Apoe4, by Thermo Fisher Scientific (1 mentions)

2 protocols using apoe2

Cytokine Secretion Assay Using Fibronectin and ApoE2

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Wells of a 96-well Maxisorp (Nunc) plate were co-coated with fibronectin (Millipore, catalog no. FC010) or ApoE2 (Peprotech, catalog no. 350-12) with human anti-KLH (each at 5 μg/mL in PBS) at room temperature for 2 hours, then washed twice with PBS and preincubated with RPMI1640 containing 10% FBS for 2 hours. THP-1 cells (2 × 10⁵ cells/well) or tolerogenic human DCs (7 × 10⁴ cells/well; generated as described in “Differentiation of primary human DCs and generation of knockout DCs”) were plated on the coated wells. In some experiments, cells were washed, resuspended in X-Vivo 15 media (Lonza, catalog no. 04-418Q), and incubated with the designated antibodies (5 μg/mL) in solution at room temperature for 20 minutes before plating onto coated wells. After overnight incubation at 37°C, culture supernatants were collected, and cytokine secretion was measured by Luminex assay using human IL8 simplex kits (Thermo Fisher Scientific, catalog no. EPX01A-10204-901, RRID: AB_2575769) or human TNFα simplex kits (Thermo Fisher Scientific, catalog no. EPX01A-10223-901, RRID: AB_2575781) according to the manufacturer's instructions.

Paavola K.J., Roda J.M., Lin V.Y., Chen P., O'Hollaren K.P., Ventura R., Crawley S.C., Li B., Chen H.I., Malmersjö S., Sharkov N.A., Horner G., Guo W., Kutach A.K., Mondal K., Zhang Z., Lichtman J.S., Song C., Rivera L.B., Liu W., Luo J., Wang Y., Solloway M.J., Allan B.B., Kekatpure A., Starck S.R., Haldankar R., Fan B., Chu C., Tang J., Molgora M., Colonna M., Kaplan D.D, & Hsu J.Y. (2021). The Fibronectin–ILT3 Interaction Functions as a Stromal Checkpoint that Suppresses Myeloid Cells. Cancer Immunology Research, 9(11), 1283-1297.

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Apolipoprotein Interactions with Lipid Nanoparticles

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LNPs and Apos were incubated in ddH₂O water overnight at 4 °C. LNPs equivalent of 5 µg encapsulated plasmid DNA were incubated with 3 µg of each of the different human Apos; ApoA1 (Sigma, SRP4693), ApoB (Sigma, 178456), ApoC3 (Sigma, A3106), ApoD (Sigma, SRP4326), ApoE2 (Peprotech, 350-12), ApoE4 (Peprotech, 350-04) and ApoH (Sigma, G9173), unless otherwise stated, in a final volume of 200 µl. For control experiments (i.e. LNP only), LNPs were incubated with ddH₂O water (in the absence of any apolipoprotein) overnight at 4 °C in a final volume of 200 µl. For Cryo TEM reactions were set up as described above in the absence of ddH₂O.

Alam S.B., Wang F., Qian H, & Kulka M. (2023). Apolipoprotein C3 facilitates internalization of cationic lipid nanoparticles into bone marrow-derived mouse mast cells. Scientific Reports, 13, 431.

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