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Recombinant human il 2 protein

Manufactured by Thermo Fisher Scientific
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Sourced in Israel

Recombinant human IL-2 protein is a cytokine produced in E. coli that stimulates the growth and differentiation of T cells and natural killer cells.

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Lab products found in correlation

Igg1κ pe, by BioLegend (2 mentions) Igg1κ fitc, by BioLegend (2 mentions) Calcein am, by Merck Group (2 mentions) Apc anti human cd56, by BioLegend (1 mentions) Igg2a apc, by BioLegend (1 mentions) Human ifn γ elisa max deluxe kit, by BioLegend (1 mentions) Anti human cd3 fitc, by BioLegend (1 mentions) Pe anti human cd56, by BioLegend (1 mentions) Fixation permeabilization solution kit with golgistop, by BD (1 mentions) Anti human ifnγ apc, by BioLegend (1 mentions) Celltiter glo luminescent cell viability assay kit, by Promega (1 mentions)

2 protocols using recombinant human il 2 protein

1

Immunophenotyping and Cytokine Quantification

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APC anti-human CD56, FITC anti-human CD3, PE anti-human CD16, murine isotype controls (IgG1κ-PE, IgG1κ-FITC, IgG2a –APC) and human IFN-γ ELISA MAX™ Deluxe kit were purchased from BioLegend Inc. (San Diego, CA). The recombinant human IL-2 protein was obtained from PeproTech (Rehovot, Israel). Calcein-AM was purchased from Sigma-Aldrich (St, Louis, MO). CCK8 kit was purchased from YESAN (Shanghai, China).
Gong C., Ni Z., Yao C., Zhu X., Ni L., Wang L, & Zhu S. (2015). A High-Throughput Assay for Screening of Natural Products that Enhanced Tumoricidal Activity of NK Cells. Biological Procedures Online, 17, 12.
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2

Natural Compound Bioactivity Assays

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20-Deoxyingenol 3-angelate (DI3A) was purchased from BioBioPha (Yunnan, China). Ingenol 3-angelate (I3A) was obtained from AdipoGen (San Diego, CA, USA). Calcein-AM was supplied by Sigma-Aldrich (St. Louis, MO, USA). Recombinant human IL-2 protein was provided by PeproTech (Rehovot, Israel). PE anti-human CD56, FITC anti-human CD107α, APC anti-human IFN-γ, and murine isotype controls (IgG1κ-PE, IgG1κ-FITC and IgG1κ–APC) were purchased from BioLegend Inc. (San Diego, CA, USA). CellTiter-Glo Luminescent Cell Viability Assay Kit was obtained from Promega (Madison, WI, USA). Fixation/Permeabilization Solution Kit with BD GolgiStop™ was provided by BD Biosciences (CA, USA).
Xu Z., Zhu X., Su L., Zou C., Chen X., Hou Y., Gong C., Ng W., Ni Z., Wang L., Yan X., Zhu Y., Jiao X., Yao C, & Zhu S. (2020). A high-throughput assay for screening natural products that boost NK cell-mediated killing of cancer cells. Pharmaceutical Biology, 58(1), 357-366.
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