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2 protocols using perchloric acid

1

Investigating Bevacizumab's Cellular Effects

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Bevacizumab (Roche Diagnostics GmbH., Mannheim, Germany, Av), acetic
acid, nitric acid, perchloric acid, sodium chloride, potassium chloride and
magnesium chloride were purchased from Guangzhou Chemical Reagent Factory.
Thiazolyl blue tetrazolium bromide (MTT), DMSO, phosphate buffer, DMEM media,
ascorbic acid, Na2SeO3, ethyleediaminetetraacetic acid
(EDTA) and 2, 7-dichlorodihydrofluorescein diacetate (DCFH-DA) were procured
from Sigma-Aldrich. EDTA-free mini protease inhibitor tablet was purchased from
Thermo Scientific. The ultrapure water from Milli-Q water purification system
(Milipore) was used in all experiments.
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2

Synthesis of Platinum-Based Catalysts

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Copper sulfate pentahydrate (CuSO 4 5H 2 O) and potassium hydroxide (KOH) were purchased from Damao Chemical Reagent Factory (Tianjin, China). Chloroplatinic acid hexahydrate (H 2 PtCl 6 6H 2 O) was purchased from Jinke Chemical Reagent Co., Ltd (Shenyang, China). Trisodium citrate dihydrate (C 6 H 5 Na 3 O 7 2H 2 O), ethylene glycol (EG), perchloric acid (HClO 4 ) and isopropanol were all supplied by Guangzhou Chemical Reagent Factory (Guangzhou, China). Nafion solution (5.0 wt%, D520) was provided by DuPont. The abovementioned reagents were of analysis grade (A.R.) and used without further purification. Commercial Pt/C (Hispec-3000, 20 wt% loading) was purchased from Johnson Matthey. Deionized water (18.2 MΩ cm) was used for preparing the solutions.
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