Absorption spectra were taken on an Agilent Technologies Cary 60 UV‐VIS spectrophotometer. Chlorophyll concentration and chlorophyll a to b ratios were determined according to Porra et al. (1989 ).
Cary 60 uv
Manufactured by Agilent Technologies
Sourced in United States
The Cary 60 UV is a highly accurate and versatile spectrophotometer designed for a wide range of applications in the ultraviolet-visible (UV-Vis) spectroscopy range. It provides precise and reliable measurements of the absorption or transmission of light through samples, enabling users to analyze the chemical and physical properties of various materials.
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8 protocols using cary 60 uv
1
Chlorophyll Quantification by Spectrophotometry
2
Chlorophyll Quantification Protocol
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Absorption spectra were taken on an Agilent Technologies Cary 60 UV‐VIS spectrophotometer. Chlorophyll concentrations and chlorophyll a/b ratios were determined according to Porra et al. (1989 ).
3
Drug Release from Fiber Capsules
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To study the in vitro release of DOX from the core/shell fiber capsules, the DOX-loaded (1%, w/v) core/shell fiber capsules were incubated in 2 mL phosphate-buffered saline (PBS, pH 7.4). At each time point, 1 mL PBS was taken to measure the concentration of DOX by UV–Vis absorption spectra (Cary 60 UV, Agilent Technologies, Santa Clara, CA, USA) at 485 nm. Then, 1 mL of fresh PBS was added to each sample.
DEX release profiles were determined by suspending drug-loaded particles (0.08 g) and core/shell fiber capsules carrying the drug-loaded microspheres (0.08 g) in 50 mL of PBS, respectively. The samples were incubated at 37 °C under agitation (50 rpm). At selected time points, the supernatants were removed and replaced with fresh buffer. The concentration of DEX in the supernatant was determined using the UV detection method described above.
DEX release profiles were determined by suspending drug-loaded particles (0.08 g) and core/shell fiber capsules carrying the drug-loaded microspheres (0.08 g) in 50 mL of PBS, respectively. The samples were incubated at 37 °C under agitation (50 rpm). At selected time points, the supernatants were removed and replaced with fresh buffer. The concentration of DEX in the supernatant was determined using the UV detection method described above.
4
Comprehensive Characterization of Colloidal Metal Hydroxide Nanoparticles
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Fourier-transform
infrared spectroscopy and UV–visible spectroscopy are undertaken
to confirm the formation of metal hydroxide colloidal NPs on the Agilent
Cary 630 FTIR spectrometer within the frequency range from 650 to
4000 cm–1 and Agilent Cary 60 UV–visible
spectrophotometer, respectively. Particle size and zeta potential
analyses are conducted on an Anton Paar’s PSA analyzer. The
morphology and surface structure of thin-film catalysts are viewed
via SEM using the Nova Nano SEM microscope (NOVA FEI SEM-450 equipped
with EDX detector). Surface and bulk compositional analysis are carried
out via EDS and XPS techniques using a NOVA FEI SEM-450 equipped with
an EDX detector and VersaProbeIII XPS (PHI 5000, ULVAC-PHI) X resource:100u25w15KV,
respectively. The active phase of the catalyst is evaluated via XRD
pattern analysis and Raman spectroscopy analysis on the Rigaku-Dmax
3C diffractometer (Rigaku Corp Tokyo Japan) with Cu Kα (λmax = 1.54056) radiation and iRaman 532 nm Raman spectrometer
(SN.17003)/iRaman, respectively. After preparations, samples were
scratched from glass substrates, and analyses (XRD and/or XPS) were
performed using powder samples only.
infrared spectroscopy and UV–visible spectroscopy are undertaken
to confirm the formation of metal hydroxide colloidal NPs on the Agilent
Cary 630 FTIR spectrometer within the frequency range from 650 to
4000 cm–1 and Agilent Cary 60 UV–visible
spectrophotometer, respectively. Particle size and zeta potential
analyses are conducted on an Anton Paar’s PSA analyzer. The
morphology and surface structure of thin-film catalysts are viewed
via SEM using the Nova Nano SEM microscope (NOVA FEI SEM-450 equipped
with EDX detector). Surface and bulk compositional analysis are carried
out via EDS and XPS techniques using a NOVA FEI SEM-450 equipped with
an EDX detector and VersaProbeIII XPS (PHI 5000, ULVAC-PHI) X resource:100u25w15KV,
respectively. The active phase of the catalyst is evaluated via XRD
pattern analysis and Raman spectroscopy analysis on the Rigaku-Dmax
3C diffractometer (Rigaku Corp Tokyo Japan) with Cu Kα (λmax = 1.54056) radiation and iRaman 532 nm Raman spectrometer
(SN.17003)/iRaman, respectively. After preparations, samples were
scratched from glass substrates, and analyses (XRD and/or XPS) were
performed using powder samples only.
5
Spectroscopic Analysis of Lipoic Acid and DTT
6
Characterization of Graphene Oxide Nanoparticles
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The X-ray diffraction studies of GO NPs were performed on a Rigaku 600Miniflex X-ray diffraction (XRD) instrument with Cu k-α radiation (λ= 1.5412) in the scanning range of 100 -800. UV–visible (UV–vis) spectrum was taken in the wavelength range of 200–600 nm using Agilent Technologies Cary 60 UV–vis to confirm the absorbance of GO NPs and to observe the changes in absorbance caused by variations in reaction conditions. Fourier transform infrared (FTIR) spectra of all samples were recorded in a range of 650–4000 cm−1 in order to identify the characteristic functional groups present on the surface of the GO. Morphological characterization of the green synthesized graphene oxide nanoparticles was performed using high-resolution scanning electron microscopy (HR-SEM).
7
Corneal Permeability Assay with Fluorescein
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The corneas used in the “Corneal Opacity” section were placed back into the Franz cells. The receptor chamber was filled in with 6 mL of PBS, while 1 mL of 0.4% (w/v) fluorescein was placed into the donor chamber. A sample of each Franz cell was collected from the receptor chamber to determine the amount of fluorescein that crossed the treated corneas at 90 min. Fluorescein concentration was measured by a spectrophotometer (Agilent® Cary 60 UV) at a wavelength of 490 nm.
8
Spectroscopic Analysis of Chemical Compounds
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An Agilent
Technologies spectrophotometer (type: Cary 60 UV–Vis), in MeOH;
λmax [nm] (εrel); 10 × 10 mm2 UV cells (quartz). 1H- and 13C-nuclear magnetic spectroscopy (NMR): Bruker (Ultrashield
600 MHz) spectrometer, δ in ppm with δ (1HDO)
= 5.00 ppm40 (link) at 5 °C, JHH (Hz); 13C signal assignment from heteronuclear 1H- and 13C-hetero single quantum correlation (HSQC)
and HMBC experiments. LC–MS: a Shimadzu HPLC
system, an LC-20AD pump, a DGU-20A5 online degasser unit, an SPD-M20A
diode array detector, a Rheodyne injection valve with a 200 μL
loop; column: Phenomenex Hyperclone column ODS 5 μm 250 ×
4.6 mm2 i.d., protected with a Phenomenex ODS 4 ×
3 mm2 i.d. precolumn; flow rate 0.5 mL min–1; solvent A: 4 mM aq ammonium acetate, solvent B: MeOH; solvent composition A/B (v/v): 0–5 min: 80/20; 5–55
min: 80/20–30/70; 55–60 min: 30/70–0/100; 60–70
min: 0/100; 70–75 min: 0/100–80/20. Data were collected
and processed using Shimadzu LC Solution software (version 1.24 SP1).
Technologies spectrophotometer (type: Cary 60 UV–Vis), in MeOH;
λmax [nm] (εrel); 10 × 10 mm2 UV cells (quartz). 1H- and 13C-nuclear magnetic spectroscopy (NMR): Bruker (Ultrashield
600 MHz) spectrometer, δ in ppm with δ (1HDO)
= 5.00 ppm40 (link) at 5 °C, JHH (Hz); 13C signal assignment from heteronuclear 1H- and 13C-hetero single quantum correlation (HSQC)
and HMBC experiments. LC–MS: a Shimadzu HPLC
system, an LC-20AD pump, a DGU-20A5 online degasser unit, an SPD-M20A
diode array detector, a Rheodyne injection valve with a 200 μL
loop; column: Phenomenex Hyperclone column ODS 5 μm 250 ×
4.6 mm2 i.d., protected with a Phenomenex ODS 4 ×
3 mm2 i.d. precolumn; flow rate 0.5 mL min–1; solvent A: 4 mM aq ammonium acetate, solvent B: MeOH; solvent composition A/B (v/v): 0–5 min: 80/20; 5–55
min: 80/20–30/70; 55–60 min: 30/70–0/100; 60–70
min: 0/100; 70–75 min: 0/100–80/20. Data were collected
and processed using Shimadzu LC Solution software (version 1.24 SP1).
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