Biotinylated anti pdgfrα

Manufactured by R&D Systems

Sourced in United States

Biotinylated anti-PDGFRα is a lab equipment product that detects the presence of platelet-derived growth factor receptor alpha (PDGFRα) in various biological samples. It utilizes biotin labeling to facilitate detection and analysis of PDGFRα, a receptor protein involved in cell growth and differentiation processes.

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Lab products found in correlation

Facsaria 2 flow cytometer, by BD (1 mentions) Collagenase type 2, by Worthington (1 mentions) M1 70, by BD (1 mentions) Fitc conjugated anti cd31, by R&D Systems (1 mentions) Facscalibur, by BD (1 mentions) Facsvantage se, by BD (1 mentions) Pe cy5 streptavidin, by BD (1 mentions) Fitc conjugated anti cd90, by BD (1 mentions) Fitc conjugated anti cd105, by BioLegend (1 mentions) Fitc conjugated anti cd34, by BD (1 mentions) Fitc conjugated anti cd45, by BD (1 mentions)

Spelling variants of this product

PDGFRα (17 citations) Anti-PDGFRα (12 citations)

2 protocols using biotinylated anti pdgfrα

Isolation and Characterization of Muscle-Derived Cell Populations

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TA, GC, and Qu muscles were used in this study. Mononuclear cells from uninjured or injured limb muscles were prepared using 0.2% collagenase type II (Worthington Biochemical) as previously described [29 (link)]. FITC-conjugated anti-CD31, -CD45, PE-conjugated anti-Sca-1, and biotinylated-SM/C-2.6 [30 (link)] antibodies were used for satellite cell staining.
For detection of macrophages or neutrophils, FITC-conjugated anti-CD45 and PE-conjugated anti-F4/80 (Clone; BM8, BioLegend) or PE-conjugated anti-CD11b (Clone; M1/70, BD Pharmingen), APC-conjugated anti-Ly6G (Clone; 1A8, BioLegend), and V450-conjugated anti-Ly6C (Clone; AL-21, BD Pharmingen) antibodies were used, respectively. For detection of mesenchymal progenitors, FITC-conjugated anti-CD31, -CD45, PE-conjugated anti-Sca-1, and biotinylated anti-PDGFRα (R&D Systems, Minneapolis, MN, USA) were used as described previously [16 (link)]. Cell sorting was performed using an FACS Aria II flow cytometer (BD Immunocytometry Systems).

Inaba S., Hinohara A., Tachibana M., Tsujikawa K, & Fukada S.I. (2018). Muscle regeneration is disrupted by cancer cachexia without loss of muscle stem cell potential. PLoS ONE, 13(10), e0205467.

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Multiparametric Characterization of Stem Cells

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Cells were trypsinized and resuspended in washing buffer consisting of PBS with 2.5% FBS, and stained with primary antibodies for 30 min at 4 °C. Cells were then stained with streptavidin-PE/Cy5 (1 : 200; BD Pharmingen, Franklin Lakes, NJ, USA) for 30 min at 4 °C in the dark. Primary antibodies used for cell staining were PE-conjugated anti-CD56 (1 : 20; Miltenyi Biotec), biotinylated anti-PDGFRα (2.5 μg/ml; R&D; cat. no. BAF322), FITC-conjugated anti-CD34 (1 : 10; BD Pharmingen), FITC-conjugated anti-CD45 (1 : 10; BD Pharmingen), FITC-conjugated anti-CD90 (1 : 200; BD Pharmingen), FITC-conjugated anti-CD105 (1 : 10; BioLegend, San Diego, CA, USA), and FITC-conjugated anti-CD166 (1 : 20; MBL, Aichi, Japan). Stained cells were analyzed by FACSCalibur or FACSVantage SE (BD Biosciences). Cell sorting was performed on a FACSVantage SE.

Uezumi A., Fukada S., Yamamoto N., Ikemoto-Uezumi M., Nakatani M., Morita M., Yamaguchi A., Yamada H., Nishino I., Hamada Y, & Tsuchida K. (2014). Identification and characterization of PDGFRα+ mesenchymal progenitors in human skeletal muscle. Cell Death & Disease, 5(4), e1186-.

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