Rs baclofen

All compounds were from Sigma-Aldrich, Poole, UK unless otherwise stated. Stock solutions of pregnenolone sulphate, CIM0216 (Tocris Bioscience; Bristol, UK), WIN 55212–2 (Tocris Bioscience; Bristol, UK), AM251 (Tocris Bioscience; Bristol, UK), Gallein (Santa Cruz Biotechnology; Heidelberg, Germany) and BIIE 0246 (Tocris Bioscience; Bristol, UK) were made in DMSO (Calbiochem; Darmstadt, Germany). Stock solutions of morphine and naloxone were made in H₂O and DAMGO (Tocris Bioscience; Bristol, UK), SB205607 (Tocris Bioscience; Bristol, UK), U50488 (Tocris Bioscience; Bristol, UK) and PYY (ABGent; San Diego, CA), were made in physiological extracellular solution. A stock solution of 8-bromo cAMP was made in H₂O titrated with NaOH. Stock solutions of L-AP4 (Tocris Bioscience; Bristol, UK) and (RS)-Baclofen (Tocris Bioscience; Bristol, UK) were made in molar equivalent solutions of NaOH. Stock solutions were aliquoted and stored at −20°C. Stock solutions were diluted in physiological extracellular solution for their use in experiments. PTX (lyophilised powder) was reconstituted in H₂O and was stored at 4°C. PTX was added to cells at a concentration of 200 ng/ml.

cDNAs for eGFP and murine GABA_AR α1, α4, β2/3, γ2L and δ subunits and GABA_BR R1a and R2 subunits, cloned into a pRK5 vector, have been described previously (Hannan et al., 2011 (link), 2019 ). CGP7930 and (RS) - baclofen were acquired from Tocris. CGP55845, and other drugs, were obtained from Merck (Sigma-Aldrich) unless otherwise stated.