96-well ELISA plates (Corning) were coated with NP2-BSA and NP27-BSA at a concentration of 10 μg/mL (Biosearch Technologies) in 1X PBS at 4ºC overnight. Plates were blocked with 5% BSA in PBS, and serum samples were plated in BSA-blocked ELISA plates and incubated at 4ºC overnight. Plates were then washed and incubated with alkaline phosphatase (AP)-coated detection antibodies (IgG1-AP [Southern Biotech], IgG2B-AP [Southern Biotech], IgG2C-AP [Southern Biotech], IgG3-AP [Southern Biotech], IgM-AP [Southern Biotech], total IgG-biotin [Southern Biotech] and streptavidin-AP). ELISA plates were developed with p-nitrophenyl phosphate buffer (Fisher Scientific) and the absorbance at 405 nm was measured with a VersaMax spectrophotometer (Molecular Devices).
Igg3 ap
Manufactured by Southern Biotech
IgG3-AP is a laboratory product that serves as an immunoglobulin G3 (IgG3) antibody conjugated with alkaline phosphatase (AP). It is a tool used in various immunoassay techniques for the detection and quantification of target analytes.
Automatically generated - may contain errors
Lab products found in correlation
Igm ap, by Southern Biotech (2 mentions)
Igg1 ap, by Southern Biotech (2 mentions)
Igg2b ap, by Southern Biotech (2 mentions)
Np2 bsa, by Biosearch Technologies (1 mentions)
Np27 bsa, by Biosearch Technologies (1 mentions)
Versamax spectrophotometer, by Molecular Devices (1 mentions)
Elisa plate, by Corning (1 mentions)
Igg2b, by Southern Biotech (1 mentions)
96 well elisa plate, by Thermo Fisher Scientific (1 mentions)
Iga ap, by Southern Biotech (1 mentions)
2 protocols using igg3 ap
1
ELISA for Antibody Quantification
2
Quantifying Murine Immunoglobulin Levels
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Sera or fecal pellets were collected from CD19-cre Usp22 KO or WT littermate mice (around 7–8 weeks old), and fecal supernatant was prepared (10% wt/vol) with PBS. The Ig isotype-specific ELISA assays were performed as previously described55 (link). Briefly, 96-well ELISA plates (NUNC) were coated with goat antibodies against mouse IgM (catalog number: 1021–01; 1/1000 dilution), IgG1 (catalog number: 1071–01; 1/1000 dilution), IgG2b (catalog number: 1090-01; 1/1000 dilution), IgG3 (catalog number: 1101-01; 1/333 dilution), or IgA (catalog number: 1040-01; 1/500 dilution) (all from SouthernBiotech), respectively, overnight at 4 °C. After blocking with 3% bovine serum albumin (BSA), 1/50-diluted sera or 1/5-diluted fecal samples were added into the wells, followed by serial fourfold dilution. The bound antibodies from sera or fecal supernatant were detected by the appropriate isotype-specific goat anti-mouse Ig that was conjugated with alkaline phosphatase (AP), IgM-AP (catalog number: 1021-04; 1/2000 dilution); IgG1-AP (catalog number: 1070-04; 1/2000 dilution); IgG2b-AP (catalog number: 1090-04; 1/2000 dilution); IgG3-AP (catalog number: 1100-04; 1/4000 dilution); IgA-AP (catalog number: 1040-04; 1/2000 dilution) (all from SouthernBiotech), followed by the development with SIGMAFAST p-Nitrophenyl phosphate substrate.
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