NMR spectra were obtained using a Bruker DRX-600 spectrometer. HR-ESI-MS were recorded on an AB SCIE x500 Q-TOF MS spectrometer (AB Sciex Pte. Ltd., MA, United States). High-performance liquid chromatography (HPLC) was carried out using a Waters2535 chromatography system (Waters, Milford, MA, United States) equipped with a Waters 2,489 UV/visible detector with a YMC-Pack ODS-A (250 × 10 mm, 5 μm) column (YMC Co., Ltd., Kyoto, Japan). Acetonitrile and water (HPLC grade) were purchased from Fisher Scientific Ltd.
Waters2535 chromatography system
Manufactured by Waters Corporation
Sourced in United States
The Waters2535 chromatography system is a high-performance liquid chromatography (HPLC) instrument designed for analytical and preparative applications. It features a quaternary solvent delivery system, an autosampler, and a photodiode array (PDA) detector. The system is capable of performing isocratic, gradient, and programmed flow rate separations. The Waters2535 is suitable for a wide range of chromatographic analyses, including small molecule, peptide, and protein separations.
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Lab products found in correlation
2 protocols using waters2535 chromatography system
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Spectroscopic Characterization of Compounds
2
Spectroscopic Characterization of Compounds
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HR-ESI-MS spectra were acquired on a Waters Xevo G2 Q-TOF mass spectrometer (Waters Co., Milford, MA, USA). NMR spectra were recorded on n a Bruker AM-600 spectrometer with TMS as an internal standard (Bruker, Ettlingen, German). NMR spectra were recorded at 25°C on a Bruker AM-600 spectrometer equipped with a cryoprobe, and deuterated solvents signal was used as an internal standard. The column chromatography (CC) was performed on YMC RP-18 gel (Fuji Silysia Chemical Ltd., Kasugai, Japan) and silica gel (200–300 mesh, Qingdao Marine Chemical Ltd., Qingdao, China). High-performance liquid chro-matography (HPLC) was performed on Waters 2,535 chromatography system (Waters, Milford, MA, USA) equipped with a Waters 2,489 UV/visible detector with a YMC-Pack ODS-A (250 × 10 mm, 5 μm) column (YMC Co., Ltd., Kyoto, Japan).
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