Reagents and solvents were commercially available and used without further purification, unless otherwise noted: sodium chloride (0.9% USP) and sterile water for Injection (USP) were purchased from Hospira; Dehydrated Alcohol for Injection (USP) was obtained from Akorn Inc. Shimalite-Nickle was purchased from Shimadzu; iodine was obtained from EMD; phosphorus pentoxide was acquired from Fluka; molecular sieves were purchased from Alltech; and HPLC columns were acquired from Phenomenex. Other synthesis components were obtained as follows: sterile filters were acquired from Millipore; C18-light Sep-Paks and Porapak Q were purchased from Waters Corporation; 10 cc sterile vials were obtained from HollisterStier. Sep-Paks were flushed with 10 mL of ethanol followed by 10 mL of sterile water prior to use.
Sterile water for injection
Manufactured by Pfizer
Sourced in United States
Sterile water for Injection is a laboratory product used as a diluent for the preparation of injectable solutions. It is a highly purified, sterile, and pyrogen-free water formulation that meets the requirements for water used in the production of injectable pharmaceutical products.
Automatically generated - may contain errors
3 protocols using sterile water for injection
1
Purification and Characterization of Reagents
2
Radiolabeled Compound Synthesis Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All the chemicals (except for reference standard 6a and precursor 7 noted above) were purchased from commercially available suppliers and used without purification: sodium chloride, 0.9% USP and Sterile Water for Injection, USP were purchased from Hospira; Dehydrated Alcohol for Injection, USP was obtained from Akorn Inc. (Lake Forest IL, USA) HPLC was performed using a Shimadzu (Kyoto, Japan) LC-2010A HT system equipped with a Bioscan B-FC-1000 radiation detector, and HPLC columns were acquired from Phenomenex (Torrance CA, USA). Other synthesis components were obtained as follows: sterile filters were acquired from MilliporeSigma (Burlington MA, USA); C18 Vac 1cc Sep-Paks were purchased from Waters Corporation (Milford MA, USA); Sep-Paks were flushed with 5 mL of ethanol followed by 10 mL of sterile water prior to use.
3
Taniborbactam and Cefepime Protocols
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Taniborbactam (batch numbers CA19-1355 and CA20-0265; Venatorx Pharmaceuticals Inc., Malvern, PA, USA) was used for in vivo and in vitro testing. For in vitro testing, taniborbactam master stock (10 mg/mL) was prepared in DMSO. For in vivo testing, taniborbactam was reconstituted to 10 mg/mL using sterile water for injection (Hospira, Inc., Lake Forest, IL, USA). Subsequent dilutions in sterile 0.9% normal saline (NS) solution (B. Braun Medical Inc., Irvine, CA, USA) were made to attain final concentrations that would deliver the required doses based on study mice population’s mean weight. Cefepime HCl (batch number LRAB8503, Sigma–Aldrich, Inc., St. Louis, MO, USA) was used for in vitro testing. Cefepime 1 g vials (lots 108307C and 108725C, WG Critical Care, LLC, Paramus, NJ, USA) were reconstituted and diluted with 0.9% NS for in vivo testing. Taniborbactam and cefepime were administered via subcutaneous injections of 0.1 mL volumes.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!