Bioanalyzer 2100 hs kit

Manufactured by Agilent Technologies

The Bioanalyzer 2100 HS kit is a lab equipment product from Agilent Technologies. It is designed for the analysis of nucleic acid samples, providing automated electrophoretic separation and detection. The kit includes reagents and consumables required for the analysis.

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Lab products found in correlation

Nextseq 550, by Illumina (1 mentions) Hiseq x, by Illumina (1 mentions) Hiseq 2500, by Illumina (1 mentions) Miseq v2 300 cycle kit, by Illumina (1 mentions) Ampure xp, by Beckman Coulter (1 mentions)

Close spelling variants of this product

2100 Bioanalyzer (8194 citations) Bioanalyzer (3289 citations) HS 2100 Bioanalyzer (3 citations) Agilent bioanalyzer 2100 HS DNA kit (2 citations)

2 protocols using bioanalyzer 2100 hs kit

Illumina-based Single-cell RNA Sequencing

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Cleaned up pooled single-cell libraries were analyzed using the Aglient Bioanalyzer 2100 HS kit. Libraries were sequenced at UBC Biomedical Research Centre (BRC) in Vancouver, British Columbia on the Illumina NextSeq 550 (mid- or high-output, paired-end 150-bp reads), or at the GSC on Illumina HiSeq2500 (paired-end 125-bp reads) and Illumina HiSeqX (paired-end 150-bp reads).

Laks E., McPherson A., Zahn H., Lai D., Steif A., Brimhall J., Biele J., Wang B., Masud T., Ting J., Grewal D., Nielsen C., Leung S., Bojilova V., Smith M., Golovko O., Poon S., Eirew P., Kabeer F., Ruiz de Algara T., Lee S.R., Taghiyar M.J., Huebner C., Ngo J., Chan T., Vatrt-Watts S., Walters P., Abrar N., Chan S., Wiens M., Martin L., Scott R.W., Underhill T.M., Chavez E., Steidl C., Da Costa D., Ma Y., Coope R.J., Corbett R., Pleasance S., Moore R., Mungall A.J., Mar C., Cafferty F., Gelmon K., Chia S., Marra M.A., Hansen C., Shah S.P, & Aparicio S. (2019). Clonal Decomposition and DNA Replication States Defined by Scaled Single-Cell Genome Sequencing. Cell, 179(5), 1207-1221.e22.

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Single-Cell RNA-seq Library Preparation

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For each triplicate condition, a total of 1000 cells were dispensed in single wells and processed using the Smart-seq2 protocol^{96 (link)}. The amount of PCR cycles for the second-strand generation step were adjusted to 12 cycles to account for the increased input material. The unfragmented libraries were quality-controlled using a Bioanalyzer 2100 HS kit (Agilent). The libraries were then processed for tagmentation, using 1 ng of amplified cDNA as input, and 8 cycles were used for the dual-indexing PCR. The DNA molarity of the libraries after AMPureXP (Beckman Coulter) clean-up was assessed using a Bioanalyzer 2100 HS and a Qubit HS (Thermo Fischer) to estimate size distribution and DNA concentration respectively. Finally, the libraries were pooled at equimolar ratios and sequenced using a MiSeq v2 300 cycle kit (Illumina) (DNA Sequencing Facility, Dept. of Biochemistry).

Kohler T.N., De Jonghe J., Ellermann A.L., Yanagida A., Herger M., Slatery E.M., Weberling A., Munger C., Fischer K., Mulas C., Winkel A., Ross C., Bergmann S., Franze K., Chalut K., Nichols J., Boroviak T.E, & Hollfelder F. (2023). Plakoglobin is a mechanoresponsive regulator of naive pluripotency. Nature Communications, 14, 4022.

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