MRI examination involved a Siemens 3.0 T MRI scanner (Siemens Healthineers), loop coil and scanning sequence, including T1 weighted imaging (T1WI), T2 weighted imaging (T2WI) and DWI sequences (Table I ), with a diffusion time of ~10 min. B (dispersion-sensitive gradient) selection was as follows (19 (link),20 (link)): 0, 500, 800, 1,000, 1,500, 2,000, 2,500 and 3,000 sec/mm2. Nude mice were anesthetized using 2% pentobarbital injection (0.05 ml/mouse) before scanning, and the tumor-bearing nude mice were wrapped in fresh pork to reduce the magnetic susceptibility artifact (21 (link)) and placed in the loop coils.
3.0t mri scanner
Manufactured by Siemens
Sourced in Germany
The 3.0T MRI scanner is a medical imaging device that uses a strong magnetic field and radio waves to generate detailed images of the inside of the human body. It operates at a magnetic field strength of 3.0 Tesla, which allows for high-resolution imaging of various bodily structures and functions.
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Lab products found in correlation
12 channel head coil, by Siemens (8 mentions)
8 channel head coil, by Siemens (3 mentions)
8 channel phased array head coil, by Siemens (3 mentions)
Eight channel phased array head coil, by Siemens (2 mentions)
Mri scanner, by Siemens (1 mentions)
16 channel head coil, by Siemens (1 mentions)
Balb c nude mice, by Charles River Laboratories (1 mentions)
Elan 9000 drc, by PerkinElmer (1 mentions)
12 channel array coil, by Siemens (1 mentions)
8 channel phased array coil, by Siemens (1 mentions)
16 channel phased array head coil, by Siemens (1 mentions)
79 protocols using 3.0t mri scanner
1
MRI Imaging of Tumor-Bearing Nude Mice
2
Acute Ischemic Stroke MRI Evaluation
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According to the American Heart Association/American Stroke Association (AHA/ASA) guideline for the early management of acute ischemic stroke (18 (link)), CT/MRI scans were performed at the time of admission prior to any treatment. The MRI of all subjects was carried out within 48 h after stroke onset, with T1WI + T2WI + DWI + T2Flair + magnetic resonance angiography (MRA) sequences, which were obtained using Siemens 3.0 T MRI scanner (Siemens AG, Munich, Germany). The BAD was diagnosed by two neurologists (XW and YL) with more than 5 years of diagnostic experience. If there was a discrepancy in their diagnoses, another senior neurologist made the final decision. The intrarater reliability of BAD was tested in 20 subjects by a single assessor with kappa (κ) coefficient equal to 0.92.
3
Magnetic Nanoparticle-Based Cell Targeting
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Streptavidin-coated polyethylene glycol (PEG)-Fe3O4 nanoparticles (Nanjing Nanoeast Biological Technology Co., Ltd., Nanjing, China) were reacted with biotin-labeled M17 and the unselected initial library separately for 1 h on a shaker to generate the M17-SPIONs and Lib-SPIONs, respectively, through a biotin-streptavidin system. The probes were gathered by magnetic separation and finally re-suspended in 3% agarose gel to the required concentration. The relaxivity of the magnetic nanoparticles was measured using a Siemens 3.0T MRI scanner (Siemens AG, Munich, Germany) when the solution was solidified. The T2 weighted imaging (T2WI) measurement parameters were as follows: Time of repetition, 3,500 ms; time of echo, 91 ms; averages, 8; and field of field, 100 mm. TR is time of repetition, TE is time of echo, FOV is field of view. Subsequently, ~1×107 target cells were incubated with M17-SPIONs and Lib-SPIONs at 37°C for 1 h. Then the cells were washed thrice with PBS and resuspended in 3% agarose gel and scanned with an MRI scanner when the solution was solidified. 293T cells were used as a negative control.
4
Multimodal Neuroimaging Protocol for Brain Analysis
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The structural and resting-state functional data of all subjects were collected by Siemens 3.0-T MRI scanner (Allegra, Siemens Medical System). To ensure the quality of the data collected, the subjects were required to lie on the MRI scanner bed throughout the scan and to remain completely motionless, eyes closed and awake. To reduce the effects of noise, earplugs were worn on both ears during MRI data acquisition. The regular axial three-dimensional T1-weighted images were acquired with a spoiled gradient recall sequence with the following imaging parameters: repetition time (TR) = 2300 ms, echo time (TE) = 2.03 ms, gap = 0 mm, slice thickness = 1 mm, flip angle = 9°, matrix size = 256 * 256, field of view (FOV) = 256 * 256 mm2, slices = 176. An echo planar imaging sequence was applied to acquire the functional images, the parameters were as follows: TR = 2000 ms, TE = 30 ms, slice thickness = 4 mm, matrix = 64 × 64, FOV = 240*240 mm2, flip angle = 90°, gap = 0.4 mm, 30 axial slices, and a total of 250 volumes were collected for each participant.
5
High-Resolution Brain Imaging Protocol
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Data were acquired with a 3.0 T Siemens MRI scanner in the Dana & David Dornsife Cognitive Neuroscience Imaging Center at the University of Southern California. A single-shot T2*-weighted gradient-echo EPI sequence was used for functional imaging acquisition with the following parameters: TR/TE/θ = 2000 ms/25 ms/90o, FOV = 192×192 mm, matrix = 64×64, and slice thickness = 3 mm. Forty-one contiguous axial slices parallel to the AC-PC line were obtained to cover the whole cerebrum and part of the cerebellum. An anatomical MRI was acquired using a T1-weighted, three-dimensional, gradient-echo pulse-sequence (MPRAGE) with TR/TE/θ = 2530 ms/3.09 ms/10o, FOV = 256×256 mm, matrix = 256×256, and slice thickness = 1 mm. Two hundred and eight sagittal slices were acquired to provide a high-resolution structural image of the whole brain.
6
3T MRI Functional and Structural Imaging Protocol
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A 3.0 T Siemens MRI scanner was used for data collection in the MRI Center at South China Normal University. The functional imaging data were collected using a single‐shot T2*‐weighted gradient‐echo EPI sequence. The following scanning parameters were used: TR = 2,000 ms, TE = 30 ms, flip angle = 90°, FOV = 224 × 224 mm, matrix size = 64 × 64, slice thickness = 3.5 mm, and number of slices = 32. Anatomical data were collected with a T1‐weighted, gradient‐echo pulse sequence. The following parameters were used: TR = 1,900 ms, TE = 2.52 ms, flip angle = 9°, FOV = 256 × 256 mm, matrix size = 256 × 256, slice thickness = 1 mm, and number of slices = 176.
7
Functional and Structural Brain Imaging
8
Functional and Anatomical Brain Imaging
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Functional and anatomical brain images were collected using a 3.0 T Siemens MRI scanner with a 12-channel head coil. Blood oxygen level-dependent (BOLD) images were acquired as 36 contiguous axial slices parallel to the AC-PC line, with whole-brain coverage, utilizing an echo planar single-shot gradient echo pulse sequence (matrix size = 64 × 64, TR = 2000 ms, TE = 25 ms, flip angle = 70°, FOV = 192 mm, voxel size = 3 × 3x3, 438 images across all runs). High-resolution anatomical images (T1-weighted magnetization prepared rapid acquisition gradient echo [MPRAGE]) were acquired for anatomical localization and spatial normalization (176 1.0 mm sagittal sliced, flip angle = 9°, matrix size = 256 × 256, FOV = 250 mm, voxel size = 1 × 1 × 1 mm).
9
fMRI Acquisition Protocol for Brain Imaging
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All the fMRI experiments were performed at National Yang‐Ming University by using a 3.0 T Siemens MRI scanner (Siemens, Erlangen, Germany) equipped with a 12‐channel head coil, and the scanning was conducted in the morning. We measured T2*‐weighted images with BOLD contrast by using a 43‐slice gradient echo‐planar imaging (EPI) sequence with a repetition time (TR) of 2500 milliseconds, an echo time (TE) of 27 milliseconds, a field of view (FoV) of 200 mm, a flip angle of 77°, a matrix size of 64 × 64, and a voxel size of 3.44 × 3.44 × 3.40 mm. During each run, 200 EPI volume images were acquired along the anterior commissure–posterior commissure plane. We acquired high‐resolution structural 192‐slice T1 images by using a three-dimentional magnetization‐prepared rapid gradient echo sequence with a TR of 2530 milliseconds, TE of 3.5 milliseconds, TI of 1100 milliseconds, FoV of 256 mm, and flip angle of 7°. For each participant, the duration of each fMRI procedure was about 15 min.
10
Multimodal Brain Imaging Protocol
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All subjects underwent MRI scans using a 3.0T MRI scanner (Siemens, Germany) after MEG recording to obtain structural T1 images. The T1‐weighted images were obtained using the following parameters: sagittal orientation, slices = 176, thickness = 1 mm, TE = 2.48 ms, TR = 1900 ms, matrix = 512 × 512, and field of view = 250 × 250 mm. Three small coils were placed in the nasion and preauricular points of the participants before scanning—the same places used in MEG recording to simultaneously register MRI and MEG data.
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