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Freezeframe software

Manufactured by Actimetrics
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FreezeFrame is a software application that captures and analyzes high-speed video frames. It provides users with the ability to record and review rapid motion events with precision.

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Lab products found in correlation

H10 11rtc, by Harvard Apparatus (4 mentions) Fear conditioning chamber, by Harvard Apparatus (2 mentions) Conditioning chamber, by Harvard Apparatus (2 mentions) Motion detection software, by Actimetrics (1 mentions) Shock chamber, by Harvard Apparatus (1 mentions) Freezeframe, by Actimetrics (1 mentions) Fear conditioning system, by Actimetrics (1 mentions) Freezeview software, by Actimetrics (1 mentions) C3334, by Intan Technologies (1 mentions) Stainless steel chambers, by Med Associates (1 mentions) Ethovision 14, by Noldus (1 mentions) Env 008, by Med Associates (1 mentions) Stainless steel drop pans, by Med Associates (1 mentions)

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FreezeFrame

48 protocols using freezeframe software

1

Contextual Fear Conditioning in Mice

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Mice were tested in one of four chambers equipped with a stainless steel floor grid designed for mice (Med Associates). The chambers were arranged in a 2 x 2 grid in an isolated room separate from the room where mice were housed. Each chamber was equipped with an overhead camera, with video signals sent to Actimetrics FreezeFrame software. Foot shock generation and auditory tones were controlled by the same software. Freezing was quantified by the Actimetrics FreezeFrame software based on frame-by-frame analysis of the video signals, digitized at 4 Hz. Testing was performed at the same time each day.
Singer B.H., Newstead M.W., Zeng X., Cooke C.L., Thompson R.C., Singer K., Ghantasala R., Parent J.M., Murphy G.G., Iwashyna T.J, & Standiford T.J. (2016). Cecal Ligation and Puncture Results in Long-Term Central Nervous System Myeloid Inflammation. PLoS ONE, 11(2), e0149136.
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2

Passive Avoidance Freezing Behavior

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The OFL test was performed as previously described60 (link). Each mouse was placed in a modified passive avoidance cage (Coulbourn Instruments, Whitehall, PA, USA) for OFL conditioning and retrieval. The freezing behavior of the observer mouse was recorded and automatically analyzed with FreezeFrame software (Actimetrics).
Kim S., Lee B., Choi J.H., Kim J.H., Kim C.H, & Shin H.S. (2017). Deficiency of a brain-specific chemokine-like molecule, SAM3, induces cardinal phenotypes of autism spectrum disorders in mice. Scientific Reports, 7, 16503.
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3

Context and Cued Fear Conditioning Protocol

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The context and cue fear conditioning assay was performed as previously described57 (link). The apparatus consists of a sound attenuated chamber with a grid floor capable of delivering an electric shock, and freezing was recorded and measured with an overhead camera and FreezeFrame software (Actimetrics), respectively. On day 1, each mouse was placed into the chamber and baseline freezing behavior was recorded during the first two min. Then, mice were exposed to 80-dB white noise for 30 s as the conditioned stimulus (CS). During the final 2 s of this noise, mice received a mild foot shock (0.5 mA) as the unconditioned stimulus (US). After 1 min, mice were subjected to another CS-US pair stimulus, and then returned to their home cages and homeroom. On day 2, each mouse was returned to the test chamber and freezing behavior was recorded for 5 min (context test). Mice were returned to their home cage and placed in a different room with reduced lighting for at least 1 h. Moreover, environmental and contextual cues were changed. For the cued test, each mouse was placed in the modified chamber for 3 min, and then exposed to the auditory CS and freezing behavior was recorded for another 3 min (cue test). Baseline freezing behavior obtained during training was subtracted from the context or cue tests to control for variability in each animal.
Zhang Y.J., Gendron T.F., Grima J.C., Sasaguri H., Jansen-West K., Xu Y.F., Katzman R.B., Gass J., Murray M.E., Shinohara M., Lin W.L., Garrett A., Stankowski J.N., Daughrity L., Tong J., Perkerson E.A., Yue M., Chew J., Castanedes-Casey M., Kurti A., Wang Z.S., Liesinger A.M., Baker J.D., Jiang J., Lagier-Tourenne C., Edbauer D., Cleveland D.W., Rademakers R., Boylan K.B., Bu G., Link C.D., Dickey C.A., Rothstein J.D., Dickson D.W., Fryer J.D, & Petrucelli L. (2016). C9ORF72 poly(GA) aggregates sequester and impair HR23 and nucleocytoplasmic transport proteins. Nature neuroscience, 19(5), 668-677.
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4

Contextual and Cued Fear Conditioning in Mice

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The last assay performed was contextual and cued fear conditioning at 93 DPI(Shors et al., 1992 (link); Weiss et al., 2020 (link)). The mice were trained in a 29.5 × 25 cm x 29.5cm chamber equipped with rods that were sized and spaced for use with mice (Coulbourn Instruments, Holliston, MA). During the training the animals received 3 tone-shock stimuli over the span of 15 minutes. 24 hours post-training the animals were placed back into the training chamber and the contextual fear in the form of freezing behavior was assessed for 6 minutes. ~4 hours after contextual fear was assessed, cued fear was sassed by placing the animals into a novel 34 × 28 × 18.5 cm chamber for 8 minutes. 180s into the test the tone from training was played for 60s. Data were recorded using FreezeFrame software (Actimetrics, Wilmette, IL).
Islam M.B., Davis BT I.V., Kando M.J., Mao Q., Procissi D., Weiss C, & Schwulst S.J. (2021). Differential Neuropathology and Functional Outcome After Equivalent Traumatic Brain Injury in Aged Versus Young Adult Mice. Experimental neurology, 341, 113714.
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5

Automated Fear Conditioning Protocol

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All experiments were performed using an automated, computerized fear conditioning chamber as previously described (Uhernik et al. 2018 (link)). In brief, the apparatus consisted of two plexiglass chambers, each with a sound-attenuating isolation cubicle equipped with a ventilation fan, a top-mounted USB camera, and a house light mounted on the side wall. Foot shocks and auditory cues were delivered through a removable floor grid by the Actimetrics FreezeFrame software. Freezing responses were captured on digital video and analyzed using motion detection software (Actimetrics).
Montoya Z.T., Uhernik A.L, & Smith J.P. (2020). Comparison of cannabidiol to citalopram in targeting fear memory in female mice. Journal of Cannabis Research, 2, 48.
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6

Contextual Fear Conditioning Protocol

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Contextual fear conditioning was conducted in four identical Plexiglas/metal chambers (25cm × 31cm × 32cm) containing metal grid floors (19 stainless steel bars, 0.5 cm in diameter, and 1.25 cm apart). All groups were counterbalanced within and across days. For conditioning, each animal was placed in the chamber for 180s (baseline freezing measurement), followed by a single 1s 1.5mA shock, followed by a 300s shock-free period (post-shock freezing measurement). Twenty-four hours later, animals were returned to the same chamber and tested for freezing to the context for 300s (retention freezing measurement). All chambers were cleaned with a 5% ammonium hydroxide solution between sessions. A camera positioned at the top of each chamber recorded behavior for each animal and transmitted the signal to a computer running FreezeFrame software (Actimetrics, Wilmette, IL). Freezeframe was configured to score freezing as 0.75s bouts without changes in pixel luminance and then verified offline by an experimenter (see Asok et al. 2014).
Asok A., Schulkin J, & Rosen J.B. (2016). Corticotropin releasing factor type-1 receptor antagonism in the dorsolateral bed nucleus of the stria terminalis disrupts contextually conditioned fear, but not unconditioned fear to a predator odor. Psychoneuroendocrinology, 70, 17-24.
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7

Investigating the Effects of IGFBP2 and IGF1 on Contextual Fear Extinction

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Testing was conducted as previously described (Burgdorf et al., 2015b (link)), and the first extinction tests occurred 1 hour postdosing. On the contextual fear training day (D0), animals were placed in a Coulbourn Instruments shock chamber (40 × 40 × 40 cm) for 400 seconds and received three 0.5-mA 1-s footshocks delivered to the floor bars at 90-, 210-, and 330-second timepoints. During extinction, rats were subjected to daily 5-minute nonreinforced (no shock) extinction trials for the first 6 days after training and on day 14 posttraining (consolidation trial). Freezing was quantified via FreezeFrame software (Actimetrics) during the last 3 minutes of each extinction trial. Animals were dosed with a single optimal dose of IGFBP2 (1 µg/kg i.v.), IGF1 (100 µg/kg i.v.), or sterile saline vehicle (1 mL/kg i.v.) 1 hour before the first extinction session (n = 9–11/group).
Burgdorf J., Colechio E.M., Ghoreishi-Haack N., Gross A.L., Rex C.S., Zhang X.L., Stanton P.K., Kroes R.A, & Moskal J.R. (2017). IGFBP2 Produces Rapid-Acting and Long-Lasting Effects in Rat Models of Posttraumatic Stress Disorder via a Novel Mechanism Associated with Structural Plasticity. International Journal of Neuropsychopharmacology, 20(6), 476-484.
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8

Fear Conditioning Protocol in Mice

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Prior to fear conditioning training, mice were acclimated to the testing room for 1 h. Mice were placed in the fear conditioning chamber (Coulbourn Instruments) for 2 min and received two pairs of a tone (2800 Hz, 85 dB, 30 s) and a co-terminating electric foot-shock (0.7 mA, 2 s) with 30 s intervals. One day after the training, mice were placed again in the chamber to test contextual fear memory for 3 min. The freezing behavior was automatically measured by Freeze Frame software (ActiMetrics, IL, USA). Data from one mouse that had freezing rate of deviation more than 2 standard deviations were excluded from the analysis.
Hwang K.D., Bak M.S., Kim S.J., Rhee S, & Lee Y.S. (2017). Restoring synaptic plasticity and memory in mouse models of Alzheimer’s disease by PKR inhibition. Molecular Brain, 10, 57.
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9

Behavioral Assessments in Mice

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Learning and memory performance was assessed using a fear conditioning paradigm. Freezing behavior was measured with an overhead camera and FreezeFrame software (Actimetrics). Open field, elevated plus maze, and light/dark exploration tests were used for the assessment of anxiety behaviors which were tracked with an overhead camera and AnyMaze software. The rotarod test was used to examine the motor coordination and motor learning in mice. See Supplemental Experimental Procedures for details.
Liu C.C., Tsai C.W., Deak F., Rogers J., Penuliar M., Sung Y.M., Maher J.N., Fu Y., Li X., Xu H., Estus S., Hoe H.S., Fryer J.D., Kanekiyo T, & Bu G. (2014). Deficiency in LRP6-mediated Wnt Signaling Contributes to Synaptic Abnormalities and Amyloid Pathology in Alzheimer’s Disease. Neuron, 84(1), 63-77.
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10

Contextual Fear Conditioning and Hippocampal Electrophysiology

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Animals were habituated to the behavioral testing facility for at least 3 d before training. Specifically, animals were transported in their home cages to a holding room separated from the testing room for 1 h/d before testing. Because mice were group-housed within their home cages and only one animal per day was tested, cagemates awaiting testing were necessarily habituated for additional days (up to 10 additional days). Mice were trained on a standard contextual FC paradigm as described previously (Neuner et al., 2015 (link)). Briefly, animals were placed in the conditioning chambers. Following a 150-s baseline period, animals received four mild foot shocks (1 s, 0.9 mA) separated by 150 ± 25 s over 10 min. The 20 s following each shock was designated as the postshock period, and freezing during each postshock period was quantified. Twenty-four hours later, animals were returned to the chambers for 10 min. Percentage time spent freezing during this time was measured using FreezeFrame software (ActiMetrics; RRID:SCR_014429) and used as an index of long-term contextual memory, consolidation and retrieval. Immediately after testing, animals were anaesthetized using isoflurane and hippocampal slices harvested for electrophysiological analysis.
Dunn A.R., Neuner S.M., Ding S., Hope K.A., O’Connell K.M, & Kaczorowski C.C. (2019). Cell-Type-Specific Changes in Intrinsic Excitability in the Subiculum following Learning and Exposure to Novel Environmental Contexts. eNeuro, 5(6), ENEURO.0484-18.2018.
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